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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제6호
- 발행연도
- 2014.1
- 수록면
- 788 - 794 (7page)
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초록· 키워드
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An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.
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참고문헌 (21)
참고문헌 신청
Almog J / 2004 / Genipin - a novel fingerprint reagent with colorimetric and fluorogenic activity / J. Forensic Sci 49 : 255 ~ 257
Bowers EM / 2007 / Salt effects on β-glucosidase: pH-profile narrowing / Biochim. Biophys. Acta 1774 : 1500 ~ 1507
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal. Biochem 72 :
Chen M / 2010 / Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementa
Chen SC / 2004 / A novel pH-sensitive hydrogel composed of N,O-carboxymethyl chitosan and alginate cross-linked by genipin for protein drug delivery / J. Control. Release 96
Bowers EM / 2007 / Salt effects on β-glucosidase: pH-profile narrowing / Biochim. Biophys. Acta 1774 : 1500 ~ 1507
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal. Biochem 72 :
Chen M / 2010 / Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementa
Chen SC / 2004 / A novel pH-sensitive hydrogel composed of N,O-carboxymethyl chitosan and alginate cross-linked by genipin for protein drug delivery / J. Control. Release 96
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