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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제8호
- 발행연도
- 2006.1
- 수록면
- 1,325 - 1,329 (5page)
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Structural modeling of β L.lactis ssp. lactis 7962 has shown that the residues Cys-472and His-509 are located in the wall of the active-site cavity.To examine the functions of Cys-472 and His-509, we generatedfive site-specific mutants: Cys-472-Ser, Cys-472-Thr, Cys-472-Met, His-509-Asn, and His-509-Phe. β-Galactosidasesubstituted at Cys-472 with Met or His-509 with Phe had<3% of the activity of the native enzyme when assayed usingONPG as substrate. The other mutants Cys-472-Ser, Cys-472-Thr, and His-509-Asn had ca. 10-15% of the nativeenzyme activity. The Vmax values of the five mutated enzymeswere lower (60-7,000-fold) than that of native enzyme.These results show that the catalytic ability of β-galactosidaseis significantly affected by mutations at Cys-472 or His-509.
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참고문헌 (16)
참고문헌 신청
Chang, H. C / 1996 / Molecular cloning of a β-D-galactosidase gene from Lactococcus lactis subsp. lactis ATCC7962 / J. Microbiol. Biotechnol. 6 : 386 ~ 390
De Vos, W. M / 1994 / Genetics of lactose utilization in lactic acid bacteria / FEMS Microbiol. Rev. 15 : 217 ~ 237
Huber, R. E / 1994 / The active site and mechanism of the beta-galactosidase from Escherichia coli / Int. J. Biochem. 26 : 309 ~ 318
Jacobson, R. H / 1994 / Three-dimensional structure of β-galactosidase from E. coli / Nature 369 : 761 ~ 766
KIM, JONG HWAN / 2005 / Characterization of the α-Galactosidase Gene from Leuconostoc mesenteroidesSY1 / Journal of Microbiology and Biotechnology 15 (4) : 800 ~ 808
De Vos, W. M / 1994 / Genetics of lactose utilization in lactic acid bacteria / FEMS Microbiol. Rev. 15 : 217 ~ 237
Huber, R. E / 1994 / The active site and mechanism of the beta-galactosidase from Escherichia coli / Int. J. Biochem. 26 : 309 ~ 318
Jacobson, R. H / 1994 / Three-dimensional structure of β-galactosidase from E. coli / Nature 369 : 761 ~ 766
KIM, JONG HWAN / 2005 / Characterization of the α-Galactosidase Gene from Leuconostoc mesenteroidesSY1 / Journal of Microbiology and Biotechnology 15 (4) : 800 ~ 808
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