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cDNA encoding somatolactin (SL) was obtained by RT-PCR from pituitary glands of rock bream (Oplegnathus fasciatus). The full length cDNA of rock bream somatolactin (rbSL) is 1636 bp long. It contains a 696 bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. rbSL has seven cysteine residues (Cys5, Cys15, Cys42, Cys65, Cys181, Cys198 and Cys206) and two potential N-glycosylation sites at positions Asn121 and Asn153. The rbSL shares 61.1～92.6% amino acid sequence similarities and 63～92.6% nucleotide sequence identities with other teleost SLs, except for goldfish and channel catfish SL. Amino acid sequence alignment revealed that rbSL has four conserved domains (ASL, BSL, CSL and DSL) common to all SLs. Out of these domains, ASL, BSL and DSL are also conserved in all teleost growth hormones and prolactins. The cDNA of rbSL has been cloned into pET expression vector in order to produce recombinant rbSL in E. coli BL21(DE3) cells. The recombinant protein showed a molecular weight of 27 kDa in SDS-PAGE.
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UCI(KEPA) : I410-ECN-0101-2009-470-014220909