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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 241 - 247 (7page)
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A new lactococcal shuttle/expression vector for lactococci, pWgal13T, was constructed using a β-galactosidase gene (lacZ) from Lactococcus lactis ssp. lactis ATCC 7962 as a screening marker. The pWgal13T was introduced into Escherichia coli DH5a and L. lactis MG1363, and was easily detected by the formation of blue colonies on a medium containing X-gal without any false transformants. Also, the quantitatively lacZ activity of pWgal13T was measured in L. lactis ssp. cremoris MG1363, and was found to be four times higher than that of L. lactis ssp. lactis ATCC7962 grown on a medium containing glucose, which shows that the lacZ gene of pWgal13T can be used for the efficient screening of L. lactis on general media. The pWgal13T was equipped with a lactococcal replicon of pWV01 from L. lactis Wg2, the new promoter P13C from L. lactis ssp. cremoris LM0230, multiple cloning sites, and a terminator for the expression of a relevant gene. The vector pWgal13T was used for the expression of the EGFP gene in E. coli and L. lactis. These results show that the lactococcal expression/shuttle vector constructed in the present study can be used for the production of foreign proteins in E. coli and L. lactis.
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참고문헌 (16)
참고문헌 신청
/ 1983 / Plasmid complements of Streptococcuslactis NCDO 712 and other lactic streptococci after protoplast-induced curing : 1 ~ 9
/ 1983 / Studies on transformationof Escherichia coli with plasmids 134 : 318 ~ 329
/ 1989 / Construction of a lactococcal expressionvector expression of hen egg white lysozyme in Lactococcuslactis subsp : 224 ~ 228
/ 1989 / cremorisgrown with glycine in osmotically Stabilized Media : 3119 ~ 3123
/ 1991 / Catabolite repression of alpha-amylasegene expression in Bacillus subtilis involves a trans-actinggene product homologous to the Escherichia coli lacI andgalR repres
/ 1983 / Studies on transformationof Escherichia coli with plasmids 134 : 318 ~ 329
/ 1989 / Construction of a lactococcal expressionvector expression of hen egg white lysozyme in Lactococcuslactis subsp : 224 ~ 228
/ 1989 / cremorisgrown with glycine in osmotically Stabilized Media : 3119 ~ 3123
/ 1991 / Catabolite repression of alpha-amylasegene expression in Bacillus subtilis involves a trans-actinggene product homologous to the Escherichia coli lacI andgalR repres
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