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Using a 50-l airlift bioreactor, for the effectiveproduction of tylosin from Streptomyces fradiae TM-224 usingfactors were studied in flask cultures. The maximum tylosinconcentration was obtained at 32oC and pH between 7.0 and7.5. When seed was inoculated after 24 h of culture, themaximum tylosin concentration, 5.7 g/l, was obtained after 4days of culture. Various concentrations of raw cornmeal weretested to investigate the optimum initial concentration for thetylosin production. An initial raw cornmeal concentration of80 g/l gave the highest tylosin concentration, 5.8 g/l, after 5days of culture. Of the various nitrogen sources, soybeanmeal and fish meal were found to be the most effective for theratio, 12 g/l of soybean meal to 14 g/l of fish meal, 7.2 g/l oftylosin was obtained after 5 days of culture. To compare rawcornmeal and glucose for the production of tylosin in the 50-lairlift bioreactor for 10 days, fed-batch cultures were carriedout under the optimum culture conditions. When raw cornmeal was used as the energy source, the tylosin productionincreased with increasing culture time. The maximum tylosinconcentration after 10 days of culture was 13.5 g/l, with aproduct yield from raw cornmeal of 0.123 g/g of consumedobtained when glucose was used as the carbon source.

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