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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 90 - 95 (6page)
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초록· 키워드
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An Actinomycetes producing an anti-VRSA (vancomycin-resistant Staphylococcus aureus) substance was isolated
from soil. The cultural, morphological, physiological and phylogenetic analyses of an isolated strain were
investigated for identification. Cultural characteristics based on ISP (International Streptomyces Project) were as
follows: white aerial mycelium, yellow reverse side, and good growth on various medium. Also, the isolate did
not produce the soluble pigment. Morphological characteristics were showed cylindrical spore chain and smooth
spore surface by SEM (Scanning Electron Microscope). Physiological characteristics were showed LL-type by
DAP isomer analysis and detected glycine, glutamic acid and alanine. A phylogenetic analysis of the 16S rDNA
provided a clue that the isolated strain was actually a member of the genus Streptomyces, because the determined
sequence exhibited a higher homology with Streptomyces echinatus. The isolate was identified to be a genus of
Streptomyces sp.. The optimal culture conditions for the maximum production of anti-VRSA substance by Streptomyces
sp. were attained in a culture medium composed of 2.0% (w/v) glucose, and 0.4% (w/v) yeast extract.
The anti-VRSA substance was highly produced after 5 days of culture. Optimal pH and temperature conditions
for the production of anti-VRSA substance were pH 7.0 and 28oC, respectively.
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참고문헌 (20)
참고문헌 신청
/ / ISCC-NBS Color-Name Charts Illustrated with CentroidColors National Bureau of Standards
/ 1967 / Use of a scanningelectron microscope for the examination of Actinomycetes 48 : 171 ~ 177
/ 1969 / Evolution of proteinmolecules : 21 ~ 132
/ 1971 / Classification ofStreptomyces spore surfaces into five groups : 527 ~ 533
/ 1979 / Two-dimensional thinlayerchromatography for amino acid analysis of bacterialcell walls : 56 ~ 58
/ 1967 / Use of a scanningelectron microscope for the examination of Actinomycetes 48 : 171 ~ 177
/ 1969 / Evolution of proteinmolecules : 21 ~ 132
/ 1971 / Classification ofStreptomyces spore surfaces into five groups : 527 ~ 533
/ 1979 / Two-dimensional thinlayerchromatography for amino acid analysis of bacterialcell walls : 56 ~ 58
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