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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제3호
- 발행연도
- 2008.1
- 수록면
- 410 - 416 (7page)
이용수
초록· 키워드
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The gene SfXyn10, which encodes a protease-resistant
xylanase, was isolated using colony PCR screening from a
genomic library of a feather-degrading bacterial strain
Streptomyces fradiae var. k11. The full-length gene consists
of 1,437 bp and encodes 479 amino acids, which includes
41 residues of a putative signal peptide at its N terminus.
The amino acid sequence shares the highest similarity
(80%) to the endo-1,4-β-xylanase from Streptomyces coelicolor
A3, which belongs to the glycoside hydrolase family 10. The
gene fragment encoding the mature xylanase was expressed
in Escherichia coli BL21 (DE3). The recombinant protein
was purified to homogeneity by acetone precipitation
and anion-exchange chromatography, and subsequently
characterized. The optimal pH and temperature for the
purified recombinant enzyme were 7.8 and 60oC, respectively.
The enzyme showed stability over a pH range of 4.0-10.0.
The kinetic values on oat spelt xylan and birchwood xylan
substrates were also determined. The enzyme activity was
enhanced by Fe2+ and strongly inhibited by Hg2+ and SDS.
The enzyme also showed resistance to neutral and alkaline
proteases. Therefore, these characteristics suggest that
SfXyn10 could be an important candidate for proteaseresistant
mechanistic research and has potential applications
in the food industry, cotton scouring, and improving animal
nutrition.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Biely, P. / 1985 / Microbial xylanolytic systems / Trends Biotechnol 3 : 286 ~ 290
Bradford, M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 72 :
Caballero, P. A. / 2007 / Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination. J. Food / Eng 81 : 42 ~ 53
Collins, T. / 2005 / Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol / Rev 29 : 3 ~ 23
Fernandez-Abalos, J. M. / 2003 / Posttranslational processing ofthe xylanase Xys1L from Streptomyces halstedii JM8 is carried out by secreted serine proteases / Microbiology
Bradford, M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 72 :
Caballero, P. A. / 2007 / Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination. J. Food / Eng 81 : 42 ~ 53
Collins, T. / 2005 / Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol / Rev 29 : 3 ~ 23
Fernandez-Abalos, J. M. / 2003 / Posttranslational processing ofthe xylanase Xys1L from Streptomyces halstedii JM8 is carried out by secreted serine proteases / Microbiology
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