지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 423 - 430 (8page)
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초록· 키워드
오류제보하기
Background: The induction of cellular defensive genes such as phase II detoxifying and antioxidant enzymes is a highly effective strategy for protection against carcinogenesis as well as slowing cancer development. Transcription factor Nrf2 (nuclear factor E2-related factor 2) is responsible for activation of phase II enzymes induced by natural chemopreventive compounds.
Methods: Red ginseng oil (RGO) was extracted using a supercritical CO₂ extraction system and chemical profile of RGO was investigated by GC/MS. Effects of RGO on regulation of the Nrf2/antioxidant response element (ARE) pathway were determined by AREeluciferase assay, western blotting, and confocal microscopy.
Results: The predominant components of RGO were 9,12-octadecadienoic acid (31.48%), bicyclo[10.1.0] tridec-1-ene (22.54%), and 22,23-dihydrostigmasterol (16.90%). RGO treatment significantly increased nuclear translocation of Nrf2 as well as ARE reporter gene activity, leading to upregulation of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase 1. Phosphorylation of the upstream kinases such as apoptosis signal-regulating kinase (ASK)1, mitogen-activated protein kinase (MAPK) kinase (MKK)4/7, c-Jun N-terminal kinase (JNK), and p38 MAPK were enhanced by treatment with RGO. In addition, RGO-mediated Nrf2 expression and nuclear translocation was attenuated by JNK inhibitor SP600125 and p38 MAPK inhibitor SB202190.
Conclusion: RGO could be used as a potential chemopreventive agent, possibly by induction of Nrf2/ARE-mediated phase II enzymes via ASK1eMKK4/7eJNK and p38 MAPK signaling pathways.
Methods: Red ginseng oil (RGO) was extracted using a supercritical CO₂ extraction system and chemical profile of RGO was investigated by GC/MS. Effects of RGO on regulation of the Nrf2/antioxidant response element (ARE) pathway were determined by AREeluciferase assay, western blotting, and confocal microscopy.
Results: The predominant components of RGO were 9,12-octadecadienoic acid (31.48%), bicyclo[10.1.0] tridec-1-ene (22.54%), and 22,23-dihydrostigmasterol (16.90%). RGO treatment significantly increased nuclear translocation of Nrf2 as well as ARE reporter gene activity, leading to upregulation of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase 1. Phosphorylation of the upstream kinases such as apoptosis signal-regulating kinase (ASK)1, mitogen-activated protein kinase (MAPK) kinase (MKK)4/7, c-Jun N-terminal kinase (JNK), and p38 MAPK were enhanced by treatment with RGO. In addition, RGO-mediated Nrf2 expression and nuclear translocation was attenuated by JNK inhibitor SP600125 and p38 MAPK inhibitor SB202190.
Conclusion: RGO could be used as a potential chemopreventive agent, possibly by induction of Nrf2/ARE-mediated phase II enzymes via ASK1eMKK4/7eJNK and p38 MAPK signaling pathways.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (55)
참고문헌 신청
[학술지(정기간행물)] - Surh YJ / 2003 / Cancer chemoprevention with dietary phytochemicals / Nat Rev Cancer 3 : 768 ~ 780
[학술지(정기간행물)] - Dorai T / 2004 / Role of chemopreventive agents in cancer therapy / Cancer Lett 215 : 129 ~ 140
[학술지(정기간행물)] - Tsao AS / 2004 / Chemoprevention of cancer / CA Cancer J Clin 54 : 150 ~ 180
[학술지(정기간행물)] - Hayes JD / 2001 / Molecular basis for the contribution of the antioxidant responsive element to cancer chemoprevention / Cancer Lett 174 : 103 ~ 113
[학술지(정기간행물)] - Nguyen T / 2009 / The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress / J Biol Chem 284 : 13291 ~ 13295
[학술지(정기간행물)] - Dorai T / 2004 / Role of chemopreventive agents in cancer therapy / Cancer Lett 215 : 129 ~ 140
[학술지(정기간행물)] - Tsao AS / 2004 / Chemoprevention of cancer / CA Cancer J Clin 54 : 150 ~ 180
[학술지(정기간행물)] - Hayes JD / 2001 / Molecular basis for the contribution of the antioxidant responsive element to cancer chemoprevention / Cancer Lett 174 : 103 ~ 113
[학술지(정기간행물)] - Nguyen T / 2009 / The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress / J Biol Chem 284 : 13291 ~ 13295
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UCI(KEPA) : I410-ECN-0101-2018-524-001598567