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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.3
- 수록면
- 103 - 114 (12page)
- DOI
- 10.1007/s13206-023-00132-0
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초록· 키워드
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Macrophages are immune cells that play important roles in the human body’s initial immune responses against pathogens and tumor cells. We investigated the use of electrical impedance monitoring to assess the differentiation of THP-1 mono- cyte into macrophages, which is necessary for immunotherapy research conducted. The change in resistance at 1 kHz and capacitance at 100 kHz measured were proportionally increased according to not only the increase in the density of resting macrophages differentiated by Phorbol-12-myristate-13-acetate treatment but also the initial number of THP-1 cells seeded on the electrode. Additionally, real-time impedance data from THP-1 cells after 48 h of cultivation demonstrated greater recognition of the resting macrophage phenotypes (adhesion cells) covered microelectrode surface with a significant increase of impedance signal in comparison with monocytes phenotypes (suspended cells). Furthermore, during the polarization phase of macrophages, the alternatively activated macrophage phenotype was larger and fl atter than that of classically activated macrophage and resting macrophage phenotypes, indicating a correlation with a higher resistance and lower capacitance impedances at 1 kHz and 100 kHz of alternatively activated macrophages (4750 Ω and – 3.5 nF) than that of classically activated macrophages (2000 Ω and – 1.5 nF) and resting macrophages (3500 Ω and – 2.0 nF), respectively. The study’s findings demonstrated that the impedance measurement system is high sensitivity and confi dence in monitoring macrophages differentiation and polarization. The electrical impedance, which has significance for each macrophage phenotype, is com- patible with macrophages characteristic features observed using flow cytometry and a microscope.
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