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논문 기본 정보

자료유형
학술저널
저자정보
Hyun Jun-Hyun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea) Woo Im-Kyung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea) Kim Kee-Tae (Research Institute, WithBio Inc., Seoul 05029, Republic of Korea) Park Young-Seo (Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea) Kang Dae-Kyung (Department of Animal Biotechnology, Dankook University, Cheonan 31116, Republic of Korea) 이나경 (Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea) Paik Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.2
발행연도
2024.2
수록면
358 - 366 (9page)
DOI
10.4014/jmb.2309.09007

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초록· 키워드

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The lactic acid bacteria, including Latilactobacillus sakei and Latilactobacillus curvatus, have been widely studied for their preventive and therapeutic effects. In this study, the underlying mechanism of action for the antioxidant and immunostimulatory effects of two strains of heat-treated paraprobiotics was examined. Heat-treated L. sakei KU15041 and L. curvatus KU15003 showed higher radical scavenging activity in both the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays than the commercial probiotic strain LGG. In addition, treatment with these two strains exhibited immunostimulatory effects in RAW 264.7 macrophages, with L. curvatus KU15003 showing a slightly higher effect. Additionally, they promoted phagocytosis and NO production in RAW 264.7 cells without any cytotoxicity. Moreover, the expression of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 was upregulated. These strains resulted in an increased expression of inducible nitric oxide synthase and cyclooxygenase-2. Moreover, the nuclear factor-κB and mitogen-activated protein kinase signaling pathways were stimulated by these strains. These findings suggest the potential of using L. sakei KU15041 and L. curvatus KU15003 in food or by themselves as probiotics with antioxidant and immune-enhancing properties.

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