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논문 기본 정보

자료유형
학술저널
저자정보
Lee Yunjeong (Department of Microbiology Chung-Ang University College of Medicine Seoul 06974 Republic of Korea) Jaikwang Nattira (Department of Microbiology Chung-Ang University College of Medicine Seoul 06974 Republic of Korea) Kim Seong keun (Department of Microbiology Chung-Ang University College of Medicine Seoul 06974 Republic of Korea) Jeong Jiseon (Department of Microbiology Chung-Ang University College of Medicine Seoul 06974 Republic of Korea) Sukhoom Ampaitip (Division of Biological Science Faculty of Science Prince of Songkla University Songkla 90110 Thailand) Kim Jong-Hwa (Department of Microbiology Chung-Ang University College of Medicine Seoul 06974 Republic of Korea) Kim Wonyong (Department of Microbiology Chung-Ang University College of Medicine Seoul 06974 Republic of Korea)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제5호
발행연도
2023.5
수록면
644 - 655 (12page)
DOI
10.4014/jmb.2211.11016

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Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain’s probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B6 biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917T, L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5’-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B6 supplementation.

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