Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system

Renyue Wei; Jiawei Lv; Xuechun Li; Yan Li; Qianqian Xu; Junxue Jin; Yu Zhang; Zhonghua Liu

추천

검색

질문

자료유형: 학술저널

저자정보: Renyue Wei (Northeast Agricultural University) Jiawei Lv (Northeast Agricultural University) Xuechun Li (Northeast Agricultural University) Yan Li (Northeast Agricultural University) Qianqian Xu (Northeast Agricultural University) Junxue Jin (Northeast Agricultural University) Yu Zhang (Northeast Agricultural University) Zhonghua Liu (Northeast Agricultural University)

저널정보: 대한수의학회 Journal of Veterinary Science Journal of Veterinary Science 제21권 제1호

발행연도: 2020.1

수록면: 84 - 98 (15page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

#iPS cell #endothelial cell #swine #mesoderm

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!