지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.9
- 수록면
- 96 - 108 (13page)
이용수
초록· 키워드
오류제보하기
Brucella is an intracellular pathogen that invades a host and settles in its immune cells; however, the mechanism of its intracellular survival is unclear. Modification of small ubiquitin-related modifier (SUMO) occurs in many cellular activities. E2 conjugating enzyme 9 (Ubc9) is the only reported ubiquitin-conjugating enzyme that links the SUMO molecule with a target protein. Brucella"s intracellular survival mechanism has not been studied with respect to SUMO-related proteins and Ubc9. Therefore, to investigate the relationship between Brucella melitensis 16M and SUMO, we constructed plasmids and cells lines suitable for overexpression and knockdown of SUMO1 and Ubc9 genes. Brucella 16M activated SUMO1/Ubc9 expression in a time-dependent manner, and Brucella 16M intracellular survival was inhibited by SUMO1/Ubc9 overexpression and promoted by SUMO1/Ubc9 depletion. In macrophages, Brucella 16M-dependent apoptosis and immune factors were induced by SUMO1/Ubc9 overexpression and restricted by SUMO1/Ubc9 depletion. We noted no effect on the expressions of SUMO1 and Ubc9 in B. melitensis 16M lipopolysaccharide-prestimulated mouse RAW264.7 macrophages. Additionally, intracellular survival of the 16MΔVirB2 mutant was lower than that of Brucella 16M (p < 0.05). VirB2 can affect expression levels of Ubc9, thereby increasing intracellular survival of Brucella in macrophages at the late stage of infection. Collectively, our results demonstrate that B. melitensis 16M may use the VirB IV secretion system of Brucella to interact with SUMO-related proteins during infection of host cells, which interferes with SUMO function and promotes pathogen survival in host cells.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host
Journal of Veterinary Science
2022 .01
-
Development and trial of vaccines against Brucella
Journal of Veterinary Science
2017 .08
-
A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
Osong Public Health and Research Persptectives
2017 .01
-
Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea
Korean Journal of Veterinary Research(대한수의학회지)
2016 .10
-
Molecular epidemiological study on a human patient of Brucella melitensis infection occurred in Korea
예방수의학회지
2019 .01
-
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Journal of Veterinary Science
2016 .03
-
몽골 유래 Brucella melitensis 동정 및 특이 SNP를 이용한 real-time PCR법에 의한 진단 평가
Korean Journal of Veterinary Research(대한수의학회지)
2015 .06
-
An Imported Case of Brucella melitensis Infection in South Korea
Infection and Chemotherapy
2018 .01
-
Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection
Journal of Veterinary Science
2018 .09
-
The in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell line
예방수의학회지
2021 .12
-
Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine
Journal of Veterinary Science
2018 .05
-
The two highly immunogenic antigens of Brucella: lipopolysaccharide (LPS) and outer membrane proteins (OMPs)
예방수의학회지
2015 .12
-
First Case of Human Brucellosis Caused by Brucella melitensis in Korea
Annals of Laboratory Medicine
2016 .01
-
Succinic acid inhibits the uptake of Brucella abortus 544 into RAW 264.7 cells and promotes survival of B. abortus in ICR mice
예방수의학회지
2021 .12
-
Protective efficacy of attenuated Salmonella Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of Brucella abortus in goats
Journal of Veterinary Science
2021 .03
-
Pathological, immunohistochemical, and bacteriological findings in dogs infected with Brucella canis
Korean Journal of Veterinary Research(대한수의학회지)
2020 .03
-
Inhibitory effect of red ginseng acidic polysaccharide from Korean red ginseng on phagocytic activity and intracellular replication of Brucella abortus in RAW 264.7 cells
Journal of Veterinary Science
2016 .09
-
Evaluation of recombinant AspC of Brucella abortus for serological diagnosis of bovine brucellosis in Korea
예방수의학회지
2015 .06
-
Review of the UBC Porcine Model of Traumatic Spinal Cord Injury
대한신경외과학회지
2018 .01
-
Properties of Synchronous Versus Metachronous Bilateral Breast Carcinoma with Long Time Follow Up
Asian Pacific journal of cancer prevention : APJCP
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-528-001324937