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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 189 - 194 (6page)
- DOI
- http://dx.doi.org/10.13041/jpvm.2015.39.4.189
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초록· 키워드
오류제보하기
The in vitro culture of Toxoplasma gondii was evaluated using a JNUCK cell strain that was independently developed by our research team. The sensitivity of the JNUCK strain was compared with those of MDCK and vero cell strains, which were previously shown to be sensitive to T. gondii. Morphological observation and 3H-uracil absorption tests showed that JNUCK cell strain has the highest sensitivity, while the MDCK and vero cell strains showed lower sensitivities. The optimal culture conditions using the JNUCK cell strain were determined by inoculating T. gondii at multiplicity of infection (MOI) 1, MOI 5, and MOI 25 and the numbers of T. gondii were measured 24, 48, and 72 hours after inoculation. The optimal medium composition was determined by adding different concentrations of FBS to DMEM medium for inoculation at MOI 5, and then calculating the numbers of tachyzoites inside the cells and in the medium. The numbers of tachyzoites inside the cell were highest for 10% FBS, while the numbers of tachyzoites in the medium peaked after addition of 30% FBS. These studies confirmed that the optimal culture condition of T. gondii using the JNUCK cell strain was achieved by inoculation at MOI 5, using medium containing 20% FBS and culturing for 72 hours.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Blader IJ / 2014 / Toxoplasma gondii development of its replicative niche: in its host cell and beyond / Eukaryot Cell 13(8):965~976
Boch J / 1974 / Toxoplasma infections in domestic animals and their importance for toxoplasmosis in humans (author's transl) / MMW Munch Med Wochenschr 116(34):1477~1480
Chi WY / 1989 / Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary’s Hospital and Jeju Medical Center / Kisaengchunghak Chapchi 27(3):171~175
Cuéllar JA / 2012 / Efficiency of in vitro culture of Toxoplasma gondii in THP1 and Vero cell lines / Biomedica 32(3):461~466
Gazzinelli RT / 2014 / Innate resistance against Toxoplasma gondii: an evolutionary tale of mice, cats, and men / Cell Host Microbe 15(2):132~138
Boch J / 1974 / Toxoplasma infections in domestic animals and their importance for toxoplasmosis in humans (author's transl) / MMW Munch Med Wochenschr 116(34):1477~1480
Chi WY / 1989 / Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary’s Hospital and Jeju Medical Center / Kisaengchunghak Chapchi 27(3):171~175
Cuéllar JA / 2012 / Efficiency of in vitro culture of Toxoplasma gondii in THP1 and Vero cell lines / Biomedica 32(3):461~466
Gazzinelli RT / 2014 / Innate resistance against Toxoplasma gondii: an evolutionary tale of mice, cats, and men / Cell Host Microbe 15(2):132~138
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