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논문 기본 정보

자료유형
학술저널
저자정보
Won Gi Yoo (Chung-Ang University College of Medicine) Sun-Min Kim (Seoul National University) Eun Jeong Won (Chonnam National University Medical School) Ji-Yun Lee (Chung-Ang University College of Medicine) Fuhong Dai (Chung-Ang University College of Medicine) Ho Choon Woo (Jeju National University) Ho-Woo Nam (Catholic University) Tae Im Kim (Chung-Ang University College of Medicine) Jeong-Hee Han (Kangwon National University) Dongmi Kwak (Kyungpook National University College of Veterinary Sciences) Yun Sang Cho (Animal and Plant Quarantine Agency) Seung-Won Kang (VexPert Co.) Tong-Soo Kim (Inha University School of Medicine) Xing-Quan Zhu (Chinese Academy) Chunren Wang (Heilongjiang Bayi Agricultural University) Heejeong Youn (Seoul National University) Sung-Jong Hong (Chung-Ang University College of Medicine)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.56 No.5
발행연도
2018.10
수록면
437 - 446 (10page)

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초록· 키워드

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To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×10³ or 1.0×10⁴ tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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