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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제16권 제1호
- 발행연도
- 2015.1
- 수록면
- 6 - 12 (7page)
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Kidney cells of canine embryos were separated into single cells using collagenase and dispase. Primary culture was conducted using these cells. To remove fibroblasts, these cells were treated with edetate disodium dihydrate (Na2EDDA), and pure epithelial cells were separated. Recombinant retrovirus particles that manifest teromerase were produced and inoculated into primary culture cells to produce immortalized canine cell strains (JNUCK-1 and JNUCK-2). To examine the characteristics of the produced cell strains, the growth curve, maximum cultured households, and expressed proteins (keratin) were identified. The JNUCK-1 and JNUCK-2 cell lines showed division ability until the 30th generation without growth retardation. JNUCK-1 and JNUCK-2 cell lines clearly expressed telomerase until the 25th generation. The canine distemper virus (CDV) was inoculated into the JNUCK-1 and JNUCK-2 cell lines, as well as in the Madin–Darby canine kidney (MDCK) cell line. The maximum titer of CDV from the JNUCK-1 cell strain was about 200 times higher than that from the MDCK cell strain. However, the JNUCK-2 cell strain produced a lower titer than the MDCK cell strain. We established a new canine kidney epithelial cell line (JNUCK-1) that could produce CDV with high titer.
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참고문헌 (20)
참고문헌 신청
Bergman J / 2006 / Comparative trial of the canine parvovirus, canine distemper virus and canine adenovirus type 2 fractions of two commercially available modified live vacc
Buczkowski H / 2014 / Morbillivirus vaccines: Recent successes and future hopes / Vaccine 32 : 3155 ~ 3161
Civiale C / 2003 / Multilayer primary epithelial cell culture from bovine conjunctiva as a model for in vitro toxicity tests / Ophthalmic Res 35 : 126 ~ 136
Grone A / 2002 / Cell Type-Dependent Cytokine Expression after Canine Distemper Virus Infection / Viral Immunol 15 : 493 ~ 505
Kaur G / 2012 / Cell lines: Valuable tools or useless artifacts / Spermatogenesis 2 : 1 ~ 5
Buczkowski H / 2014 / Morbillivirus vaccines: Recent successes and future hopes / Vaccine 32 : 3155 ~ 3161
Civiale C / 2003 / Multilayer primary epithelial cell culture from bovine conjunctiva as a model for in vitro toxicity tests / Ophthalmic Res 35 : 126 ~ 136
Grone A / 2002 / Cell Type-Dependent Cytokine Expression after Canine Distemper Virus Infection / Viral Immunol 15 : 493 ~ 505
Kaur G / 2012 / Cell lines: Valuable tools or useless artifacts / Spermatogenesis 2 : 1 ~ 5
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