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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 866 - 875 (10page)
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초록· 키워드
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COPI vesicles are essential to the retrograde transport of proteins in the early secretory pathway. The COPI coatomer complex consists of seven subunits, termed α-, β-, β′-, γ-, δ-, ε-, and ζ-COP, in yeast and mammals. Plant genomes have homologs of these subunits, but the essentiality of their cellular functions has hampered the functional characterization of the subunit genes in plants. Here we have employed virus-induced gene silencing (VIGS) and dexamethasone (DEX)-inducible RNAi of the COPI subunit genes to study the in vivo functions of the COPI coatomer complex in plants. The β′-, γ-, and δ-COP subunits localized to the Golgi as GFP-fusion proteins and interacted with each other in the Golgi. Silencing of β′-, γ-, and δ-COP by VIGS resulted in growth arrest and acute plant death in Nicotiana benthamiana, with the affected leaf cells exhibiting morphological markers of programmed cell death.
Depletion of the COPI subunits resulted in disruption of the Golgi structure and accumulation of autolysosome-like structures in earlier stages of gene silencing. In tobacco BY-2 cells, DEX-inducible RNAi of β′-COP caused aberrant cell plate formation during cytokinesis. Collectively, these results suggest that COPI vesicles are essential to plant growth and survival by maintaining the Golgi apparatus and modulating cell plate formation.
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등록된 정보가 없습니다.
참고문헌 (80)
참고문헌 신청
Ahn, C. S. / 2011 / The PP2A regulatory subunit Tap46, a component of the TOR signaling pathway, modulates growth and metabolism in plants / Plant Cell 23:185~209
Avila-Ospina, L. / 2014 / Autophagy, plant senescence, and nutrient recycling / J. Exp. Bot 65:3799~3811
Assaad, F. F. / 2001 / Plant cytokinesis. Exploring the links / Plant Physiol 126:509~516
Bassham, D. C. / 2007 / Plant autophagy-more than a starvation response / Curr. Opin. Plant Biol 10:587~593
Bassham, D. C. / 2008 / The secretory system of Arabidopsis / The Arabidopsis Book 6
Avila-Ospina, L. / 2014 / Autophagy, plant senescence, and nutrient recycling / J. Exp. Bot 65:3799~3811
Assaad, F. F. / 2001 / Plant cytokinesis. Exploring the links / Plant Physiol 126:509~516
Bassham, D. C. / 2007 / Plant autophagy-more than a starvation response / Curr. Opin. Plant Biol 10:587~593
Bassham, D. C. / 2008 / The secretory system of Arabidopsis / The Arabidopsis Book 6
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