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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제1호
- 발행연도
- 2016.1
- 수록면
- 20 - 27 (8page)
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초록· 키워드
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Lactobacillus kefiranofaciens ZW3 was obtained from kefir grains, which have high lactose hydrolytic activity. In this study, a heterodimeric LacLM-type β-galactosidase gene (lacLM) from ZW3 was isolated, which was composed of two overlapping genes, lacL (1,884 bp) and lacM (960 bp) encoding large and small subunits with calculated molecular masses of 73,620 and 35,682 Da, respectively. LacLM, LacL, and LacM were expressed in Escherichia coli BL21(DE3) and these recombinant proteins were purified and characterized. The results showed that, compared with the recombinant holoenzyme, the recombinant large subunit exhibits obviously lower thermostability and hydrolytic activity. Moreover, the optimal temperature and pH of the holoenzyme and large subunit are 60oC and 7.0, and 50oC and 8.0, respectively. However, the recombinant small subunit alone has no activity. Interestingly, the activity and thermostability of the large subunit were greatly improved after mixing it with the recombinant small subunit. Therefore, the results suggest that the small subunit might play an important role in maintaining the stability of the structure of the catalytic center located in the large subunit.
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참고문헌 (26)
참고문헌 신청
Bradford MM / 1976 / Arapid and s ensitive m ethod for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Chen W / 2009 / Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk / J. Dairy Sci 92:491~498
Corral JM / 2006 / Cloning and characterization of a β-galactosidase encoding region in Lactobacillus coryniformis CECT 5711 / Appl. Microbiol. Biotechnol 73:640~646
Dong Q / 2014 / Characterization of an extremely thermostable but cold-adaptive β-galactosidase from the hyperthermophilic archaeon Pyrococcus furiosus for use as a recombinant aggregation for batch lactose degradation at high temperature / J. Biosci. Bioeng 117:706~710
Harrington LK / 2008 / Are-appraisal of lactose intolerance / Int. J. Clin. Pract 62:1541~1546
Chen W / 2009 / Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk / J. Dairy Sci 92:491~498
Corral JM / 2006 / Cloning and characterization of a β-galactosidase encoding region in Lactobacillus coryniformis CECT 5711 / Appl. Microbiol. Biotechnol 73:640~646
Dong Q / 2014 / Characterization of an extremely thermostable but cold-adaptive β-galactosidase from the hyperthermophilic archaeon Pyrococcus furiosus for use as a recombinant aggregation for batch lactose degradation at high temperature / J. Biosci. Bioeng 117:706~710
Harrington LK / 2008 / Are-appraisal of lactose intolerance / Int. J. Clin. Pract 62:1541~1546
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