Expression and Preliminary Functional Profiling of the let-7 Family during Porcine Ovary Follicle Atresia

Rui Cao; Wang Jun Wu; Xiao Long Zhou; Peng Xiao; Yi Wang; Honglin Liu

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자료유형: 학술저널

저자정보: Rui Cao (Nanjing Agricultural University) Wang Jun Wu (Nanjing Agricultural University) Xiao Long Zhou (Nanjing Agricultural University) Peng Xiao (Nanjing Agricultural University) Yi Wang (Nanjing Agricultural University) Honglin Liu (Nanjing Agricultural University)

저널정보: 한국분자세포생물학회 Molecules and Cells Molecules and Cells 제38권 제4호

발행연도: 2015.4

수록면: 304 - 311 (8page)

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Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let- 7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

#apoptosis #follicle atresia #let-7 #MicroRNA #MiRNA #pig #stem-loop

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참고문헌 신청

Bortul, R. / 2003 / Constitutively active Akt1 protects HL60 leukemia cells from TRAIL-induced apoptosis through a mechanism involving NFkappaB activation and cFLIP(L)up-regulation / Leukemia 17:379~389

Carletti, M. Z. / 2010 / MicroRNA 21 blocks apoptosis in mouse periovulatory granulosa cells / Biol. Reprod 83:286~295

Carson, R. S. / 1979 / Gonadotropin receptors of the ovine ovarian follicle during follicular growth and atresia / Biol. Reprod 21:75~87

Chen, C. / 2005 / Real-time quantification of microRNAs by stem–loop RT–PCR / Nucleic Acids Res 33:179

Cheng, Y. / 2008 / Changes in expression and localization of X-linked inhibitor of apoptosis protein(XIAP)in follicular granulosa cells during atresia in porcine ovaries / J. Reprod. Dev 54:454~459

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