지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.2
- 수록면
- 138 - 144 (7page)
- DOI
- 10.14348/molcells.2015.2193
이용수
초록· 키워드
오류제보하기
Raloxifene is a selective estrogen receptor modulator (SERM) that binds to the estrogen receptor (ER), and exhibits potent anti-tumor and autophagy-inducing effects in breast cancer cells. However, the mechanism of raloxifeneinduced cell death and autophagy is not well-established.
So, we analyzed mechanism underlying death and autophagy induced by raloxifene in MCF-7 breast cancer cells.
Treatment with raloxifene significantly induced death in MCF-7 cells. Raloxifene accumulated GFP-LC3 puncta and increased the level of autophagic marker proteins, such as LC3-II, BECN1, and ATG12-ATG5 conjugates, indicating activated autophagy. Raloxifene also increased autophagic flux indicators, the cleavage of GFP from GFP-LC3 and only red fluorescence-positive puncta in mRFP-GFP-LC3- expressing cells. An autophagy inhibitor, 3-methyladenine (3-MA), suppressed the level of LC3-II and blocked the formation of GFP-LC3 puncta. Moreover, siRNA targeting BECN1 markedly reversed cell death and the level of LC3-II increased by raloxifene. Besides, raloxifene-induced cell death was not related to cleavage of caspases-7, -9, and PARP. These results indicate that raloxifene activates autophagy- dependent cell death but not apoptosis. Interestingly, raloxifene decreased the level of intracellular ade-nosine triphosphate (ATP) and activated the AMPK/ULK1 pathway. However it was not suppressed the AKT/mTOR pathway. Addition of ATP decreased the phosphorylation of AMPK as well as the accumulation of LC3-II, finally attenuating raloxifene-induced cell death.
Our current study demonstrates that raloxifene induces autophagy via the activation of AMPK by sensing decreases in ATP, and that the overactivation of autophagy promotes cell death and thereby mediates the anti-cancer effects of raloxifene in breast cancer cells.
목차
등록된 정보가 없습니다.
참고문헌 (39)
참고문헌 신청
Alers, S. / 2012 / Role of AMPK-mTOR-Ulk1/2 in the regulation of autophagy : cross talk, shortcuts, and feedbacks / Mol. Cell. Biol 32:2~11
Balgi, A. D. / 2009 / Screen for chemical modulators of autophagy reveals novel therapeutic inhibitors of mTORC1 signaling / PLoS One 4:7124
Bursch, W. / 1996 / Active cell death induced by the anti-estrogens tamoxifen and ICI 164 384 in human mammary carcinoma cells(MCF-7)in culture : the role of autophagy / Carcinogenesis 17:1595~1607
de Medina, P. / 2009 / Ligands of the antiestrogen-binding site induce active cell death and autophagy in human breast cancer cells through the modulation of cholesterol metabolism / Cell Death Differ 16:1372~1384
Deli, T. / 2008 / Extracellular ATP and cancer : an overview with special reference to P2 purinergic receptors / Pathol. Oncol. Res 14:219~231
Balgi, A. D. / 2009 / Screen for chemical modulators of autophagy reveals novel therapeutic inhibitors of mTORC1 signaling / PLoS One 4:7124
Bursch, W. / 1996 / Active cell death induced by the anti-estrogens tamoxifen and ICI 164 384 in human mammary carcinoma cells(MCF-7)in culture : the role of autophagy / Carcinogenesis 17:1595~1607
de Medina, P. / 2009 / Ligands of the antiestrogen-binding site induce active cell death and autophagy in human breast cancer cells through the modulation of cholesterol metabolism / Cell Death Differ 16:1372~1384
Deli, T. / 2008 / Extracellular ATP and cancer : an overview with special reference to P2 purinergic receptors / Pathol. Oncol. Res 14:219~231
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Autophagy and Oral Cancer
생명과학회지
2017 .08
-
Overview of the Minireviews on Autophagy
Molecules and Cells
2018 .01
-
Induction of cytoprotective autophagy by morusin via AMP-activated protein kinase activation in human non-small cell lung cancer cells
Nutrition Research and Practice
2020 .10
-
Induction of cytoprotective autophagy by morusin via AMP-activated protein kinase activation in human non-small cell lung cancer cells
Nutrition Research and Practice
2020 .10
-
The autophagy research in electron microscopy
한국현미경학회지
2019 .01
-
Effects of in vitro vitamin D treatment on function of T cells and autophagy mechanisms in high-fat diet-induced obese mice
Nutrition Research and Practice
2021 .12
-
Effects of in vitro vitamin D treatment on function of T cells and autophagy mechanisms in high-fat diet-induced obese mice
Nutrition Research and Practice
2021 .12
-
Copper Ion from Cu2O Crystal Induces AMPK-Mediated Autophagy via Superoxide in En-dothelial Cells
Molecules and Cells
2016 .03
-
Lipopolysaccharide (LPS)-Induced Autophagy Is Responsible for Enhanced Osteoclastogenesis
Molecules and Cells
2017 .11
-
PKA regulates autophagy through lipolysis during fasting
Molecules and Cells
2024 .01
-
The Role of Autophagy in Systemic Metabolism and Human-Type Diabetes
Molecules and Cells
2018 .01
-
Ginsenoside Rg1 Induces Autophagy in Colorectal Cancer through Inhibition of the Akt/mTOR/p70S6K Pathway
Journal of Microbiology and Biotechnology
2024 .04
-
Autophagy and Longevity
Molecules and Cells
2018 .01
-
Calcineurin Activation Mediated by Human Prion Protein Induced Neuron Cell Death via Autophagy Flux
한국미생물학회 학술대회논문집
2019 .04
-
Can Hinokitiol Kill Cancer Cells? Alternative Therapeutic Anticancer Agent via Autophagy and Apoptosis
대한임상검사과학회지
2019 .01
-
Biological Roles of Alternative Autophagy
Molecules and Cells
2018 .01
-
Autophagy induced by H. pylori VacA regulated the survival mechanism of the SGC7901 human gastric cancer cell line
Genes & Genomics
2021 .10
-
PARP1 Impedes SIRT1-Mediated Autophagy during Degeneration of the Retinal Pigment Epithelium under Oxidative Stress
Molecules and Cells
2020 .01
-
Roles of Apoptosis Related Genes in Atg8-mediated Autophagy
한국미생물학회 학술대회논문집
2020 .10
-
Novel miR-1958 Promotes Mycobacterium tuberculosis Survival in RAW264.7 Cells by Inhibiting Autophagy Via Atg5
Journal of Microbiology and Biotechnology
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0