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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 87 - 95 (9page)
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초록· 키워드
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Characterization of green tissue-specific promoters helps facilitate genetic improvement in crops. Here, we isolated a novel green tissue-specific expression gene Psak encoding photosystem I protein in rice through RTPCR analysis. The 5′ flanking region from ?635 to +60 (transcription start site of Psak as +1) covering parts of Psak gene and its promoter Ppsak was identified as be critical for green tissue-specific expression (including leaves and stems) in rice. Further promoter deletion analyses demonstrated that the promoter region from ?559 to ?232 was necessary and sufficient for green tissue-specific expression of Psak gene. Histochemical assays showed that the GUS expression of this 267-bp region was highly presented in leaves and stems, but reduced or absent in other tissues examined. The GUS expression level of this core promoter region could be 14.6- to 21.6-fold higher than that of the full-length region of Ppsak promoter in leaves and stems.
These results combined with cis-acting elements prediction in Ppsak promoter suggested that the TCT motif located in this promoter played a key role in strong green tissue-specific expression.
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참고문헌 (53)
참고문헌 신청
Bakhsh A / 2011 / Rubisco small subunit as a strong, green tissue-specific promoter / Arch Biol Sci 63:299~307
Baloch FS / 2011 / Rubisco small subunit as a strong, green tissue-specific promoter / Arch Biol Sci 63:299~307
Barton KA / 1988 / Bacillus thuringiensis δ-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to Lepidopteran insects / Plant Physiol 85:1103~1109
Block A / 1990 / Functional borders, genetic fine structure, and distance requirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter / Proc Natl Acad Sci 87:5387~5391
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Baloch FS / 2011 / Rubisco small subunit as a strong, green tissue-specific promoter / Arch Biol Sci 63:299~307
Barton KA / 1988 / Bacillus thuringiensis δ-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to Lepidopteran insects / Plant Physiol 85:1103~1109
Block A / 1990 / Functional borders, genetic fine structure, and distance requirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter / Proc Natl Acad Sci 87:5387~5391
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
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