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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.48 No.2
- 발행연도
- 2021.6
- 수록면
- 353 - 365 (13page)
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초록· 키워드
오류제보하기
Previously, we reported that tissue factor (Tf) was included in the list of differentially expressed genes as an upregulated gene in a rejected porcine heart after xenotransplantation into monkey. In this study, we analyzed that expression of Tf in aortic endothelial cells (pAEC) isolated from alpha 1,3-galactosyltransferase knockout pig in response to allogeneic porcine serum and xenogeneic human serum. The consequence was significant upregulation of Tf expression by responding to human serum compared with porcine serum. To analyze the function of Tf gene as a promoter, we constructed reporter vectors for expression of luciferase linked to 1,246 and 787 base pairs of porcine Tf (pTF1246 and pTF787), and 535 base pairs of human TF (hTF535) sequences including putative promoter regions and AP-1 biding site at the 5’ end. The reporter vectors were transfected into pAEC including cytomegalovirus enhancer/chicken β-actin (CAG)-luciferase vector as a control. Luciferase assay showed that all of the promoters were insufficient to express luciferase compared with CAG promoter in basic culture conditions. Notably, pTF1246, pTF787, and hTF535 led to a significant increase of luciferase expression in response to human serum compared with porcine serum while no change of CAG. pTF1246 and pTF787 showed higher expression than hTF535. Taken together, our findings suggest that pTF1246 and pTF787 promoters could mediate target gene expression specifically at xenogeneic stress condition.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (42)
참고문헌 신청
Ahrens HE / 2015 / siRNA mediated knockdown of tissue factor expression in pigs for xenotransplantation / American Journal of Transplantation 15:1407~1414
Aigner B / 2010 / Transgenic pigs for xenotransplantation : Selection of promoter sequences for reliable transgene expression / Current Opinion in Organ Transplantation 15:201~206
Alexopoulou AN / 2008 / The CMV early enhancer/chicken beta actin(CAG)promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors / BMC Cell Biology 9:1~11
Bennet W / 2000 / Damage to porcine islets of Langerhans after exposure to human blood in vitro, or after intraportal transplantation to cynomologus monkeys : Protective effects of sCR1 and heparin / Transplantation 69:711~719
Cowan PJ / 2003 / Targeting gene expression to endothelium in transgenic animals : A comparison of the human ICAM-2, PECAM-1 and endoglin promoters / Xenotransplantation 10:223~231
Aigner B / 2010 / Transgenic pigs for xenotransplantation : Selection of promoter sequences for reliable transgene expression / Current Opinion in Organ Transplantation 15:201~206
Alexopoulou AN / 2008 / The CMV early enhancer/chicken beta actin(CAG)promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors / BMC Cell Biology 9:1~11
Bennet W / 2000 / Damage to porcine islets of Langerhans after exposure to human blood in vitro, or after intraportal transplantation to cynomologus monkeys : Protective effects of sCR1 and heparin / Transplantation 69:711~719
Cowan PJ / 2003 / Targeting gene expression to endothelium in transgenic animals : A comparison of the human ICAM-2, PECAM-1 and endoglin promoters / Xenotransplantation 10:223~231
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