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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 359 - 367 (9page)
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초록· 키워드
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Photoperiod (Ppd) genes play an important role in the adaptation of wheat to the ecological environment. However, the transcriptional regulation mechanism of photoperiodic genes has remained elusive. This study isolated a full-length promoter of Ppd-D1b (2518?bp) from the common wheat genome. Several essential core cis-acting elements and numerous light-responsive cis-acting regulatory elements were identifed in Ppd-D1b promoter by the in-silico analysis. Ten 5’-deleted length fragments of the Ppd-D1b promoter fused with GUS were constructed and named D0 ~D9, then transferred them into Arabidopsis thaliana. GUS gene driven by full-length (D0) in transgenic Arabidopsis thaliana showed the same rhythm with Ppd-D1b in wheat under short-day conditions (SDs, 8-h light/16-h dark). The expression of GUS gene in D0 reached its peak at?3?h after dawn, then decreased to the lowest and remained stable. Analysis of the series of 5’-deleted fragments showed that at 3?h after dawn, GUS gene expression activity decreased signifcantly in D7a due to removal of CHEBS (CCA1 HIKING EXPEDITION binding site). Moreover, yeast one-hybrid (Y1H) and dual-luciferase (dual-LUC) assays revealed that TaTCP20 could bind to the Ppd-D1b promoter to increase its transcriptional activity. This study revealed a transcription factor, TaTCP20, which activated Ppd-D1b by binding to CHEBS, provided a foundation for the theoretical research on wheat’s photoperiodic response mechanism.
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등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Bainor A / 2011 / Bicinchoninic acid (BCA) assay in low volume / Anal Biochem 410:310~312
Beales J / 2007 / A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.) / Theor Appl Genet 115:721~733
Belbin FE / 2019 / Plant circadian rhythms regulate the effectiveness of a glyphosate-based herbicide / Nat Commun 10(1):3704
Bienko M / 2005 / Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis / Science 310:1821~1824
Chakrabarti B / 2011 / Impact of temperature on phenology and pollen sterility of wheat varieties / Aust J Crop Sci 5:1039~1043
Beales J / 2007 / A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.) / Theor Appl Genet 115:721~733
Belbin FE / 2019 / Plant circadian rhythms regulate the effectiveness of a glyphosate-based herbicide / Nat Commun 10(1):3704
Bienko M / 2005 / Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis / Science 310:1821~1824
Chakrabarti B / 2011 / Impact of temperature on phenology and pollen sterility of wheat varieties / Aust J Crop Sci 5:1039~1043
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