Identification and characterization of the promoter of a gene expressing mainly in the tapetum tissue of cotton (Gossypium hirsutum L.)

Kumar Paritosh; Amarjeet Kumar Singh; Amita Kush Mehrotra; Deepak Pental; Pradeep Kumar Burma

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자료유형: 학술저널

저자정보: Kumar Paritosh (University of Delhi South Campus) Amarjeet Kumar Singh (University of Delhi South Campus) Amita Kush Mehrotra (University of Delhi South Campus) Deepak Pental (University of Delhi South Campus) Pradeep Kumar Burma (University of Delhi South Campus)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제12권 제6호

발행연도: 2018.12

수록면: 377 - 388 (12page)

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Anther and tapetum-specific genes are important for understanding male gametophyte development, as well as for their use in the development of barnase/barstar-gene based male sterility and restorer system for hybrid seed production. An essential component of the system is the availability of tapetum-specific promoters. In the present study, anther-specific genes were identified in cotton using microarray-based differential expression analysis, some of which show expression specific to the anthers at a stage where tapetum tissue was fully developed. Validation of the identified genes using RT-PCR and in situ hybridization identified one novel gene (AEG?Anther Expressing Gene) encoding a putative lipid binding protein as having a tapetum-specific expression. Further, three paralogs of the gene were identified in the cotton genome out of which the gene AEG1 (Anther Expressing Gene1) was found to express in the tapetum layer. Analysis of transgenic plants developed in cotton using 1.5 Kb promoter region of the of AEG1 gene fused upstream to the reporter gene β-glucuronidase revealed a broad window of expression of the AEG1 promoter in the tapetum tissue from the tetrad stage of anthers till the degeneration of the tapetum cells. Low levels of expression were also observed in the root tissues. Expression was not observed in the stem and leaves. The broad window of expression of AEG1 promoter in the tapetum tissue makes it a suitable candidate for the expression of the barstar gene for effective fertility restoration in the barnase/barstar system.

#Anther-specific promoter #Tapetum #Differential gene expression #Reporter gene expression

참고문헌 (52)

참고문헌 신청

Amagai M / 2003 / Identification of anther-specific genes in a cruciferous model plant, Arabidopsis thaliana, by using a combination of Arabidopsis macroarray and mRNA derived from Brassica oleracea / Sex Plant Rep 15:213~220

Ariizumi T. / 2002 / Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana / Plant Cell Reports 21(1):90~96

Vincent Arondel / 2000 / Lipid transfer proteins are encoded by a small multigene family in Arabidopsis thaliana / Plant Science 157(1):1~12

S. Bhullar / 2003 / Strategies for Development of Functionally Equivalent Promoters with Minimum Sequence Homology for Transgene Expression in Plants: cis-Elements in a Novel DNA Context versus Domain Swapping / PLANT PHYSIOLOGY 132(2):988~998

Naveen Chandra Bisht / 2004 / A two gene – two promoter system for enhanced expression of a restorer gene (barstar) and development of improved fertility restorer lines for hybrid seed production in crop plants / Molecular Breeding 14(2):129~144

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