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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 364 - 371 (8page)
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The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.
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참고문헌 (36)
참고문헌 신청
Park J-I / 2006 / Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.) / Plant Cell Reports 25(5):466~474
Rai M / 2007 / Variable T-DNA linkage configuration affects inheritance of carotenogenic transgenes and carotenoid accumulation in transgenic indica rice / Plant Cell Reports 26:1221~1231
Ray K / 2007 / Development of barnase/barstar transgenic for hybrid seed production in Indian oilseed mustard (Brassica juncea L. Czern and Coss)using a mutant acetolactate synthase gene conferring resistance to imidazolinone-based herbicide ‘Persuit’ / Current Science 93:1390~1396
Rawat P / 2011 / Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics / Journal of Biosciences 36(2):363~376
Rosellini D / 2001 / Characterization of transgenic male sterility in alfalfa / Euphytica 118(3):313~319
Rai M / 2007 / Variable T-DNA linkage configuration affects inheritance of carotenogenic transgenes and carotenoid accumulation in transgenic indica rice / Plant Cell Reports 26:1221~1231
Ray K / 2007 / Development of barnase/barstar transgenic for hybrid seed production in Indian oilseed mustard (Brassica juncea L. Czern and Coss)using a mutant acetolactate synthase gene conferring resistance to imidazolinone-based herbicide ‘Persuit’ / Current Science 93:1390~1396
Rawat P / 2011 / Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics / Journal of Biosciences 36(2):363~376
Rosellini D / 2001 / Characterization of transgenic male sterility in alfalfa / Euphytica 118(3):313~319
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