지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Transcriptional regulators that belong to the PadR family play a critical role in the regulation of various biologicalprocesses, such as detoxification, catabolism, toxin production, and antibiotic resistance. The structural basis of effectorand DNA recognition by PadR has recently been provided through our previous structural study. However, it is unclearwhether the mechanism is universally used by other PadR family members. As a first step to reveal the transcriptionregulatory mechanism of Bacillus cereus PadR-like protein (bcPLP), we expressed the bcPLP protein in Escherichia colicells and purified it to homogeneity by chromatographic methods. bcPLP was crystallized in PEG 3350 solutions. A bcPLPcrystal diffracted X-ray to 1.95A resolution. Our analysis of the X-ray diffraction data indicates that the crystal belongs tospace group P21212 and has two bcPLP chains in the asymmetric unit.
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참고문헌 (16)
참고문헌 신청
Barthelmebs, L / 2000 / Knockout of the p-coumarate decarboxylase gene from Lactobacillus plantarum reveals the existence of two other inducible enzymatic activities involved in phenolic acid metabolism / Appl Environ Microbiol 66:3368~3375
De Silva, R. S / 2005 / Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily / J Biol Chem 280:13779~13783
Degrassi, G / 1995 / Purification and characterization of ferulate and p-coumarate decarboxylase from Bacillus pumilus / Appl Environ Microbiol 61:326~332
Finn, R. D / 2016 / The Pfam protein families database:towards a more sustainable future / Nucleic Acids Res 44:D279~D285
Florez, A. B / 2015 / Transcriptional regulation of mithramycin biosynthesis in Streptomyces argillaceus : dual role as activator and repressor of the PadR-like regulator MtrY / Microbiology 161:272~284
De Silva, R. S / 2005 / Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily / J Biol Chem 280:13779~13783
Degrassi, G / 1995 / Purification and characterization of ferulate and p-coumarate decarboxylase from Bacillus pumilus / Appl Environ Microbiol 61:326~332
Finn, R. D / 2016 / The Pfam protein families database:towards a more sustainable future / Nucleic Acids Res 44:D279~D285
Florez, A. B / 2015 / Transcriptional regulation of mithramycin biosynthesis in Streptomyces argillaceus : dual role as activator and repressor of the PadR-like regulator MtrY / Microbiology 161:272~284
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