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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Toxin?antitoxin (TA) systems are ubiquitous among most of prokaryotes and govern the cell death or growth arrest inresponse to environmental cues. TA systems are associated with adaptation of pathogens to unfavorable environments,indicating their potential as a target for antibiotics. Here, we purified and crystallized the TA complex from the extremophileDeinococcus radiodurans. The TA complex (DrMazEF) was co-expressed and pulled using the N-terminal glutathioneS-transferase-tagged DrMazF. The complex was crystallized in 100 mM citric acid pH 3.5 containing 25% PEG3350. Thecrystal diffracted X-ray to a 2.6 A resolution and belonged to the space group P212121, with the unit cell parameters a =46.01, b = 74.04, and c = 138.26 A. The asymmetric unit of the crystal had six molecules in two heterotrimeric complexeswith a calculated Mathew’s coefficient of 1.84 A3 Da-1 and a solvent content of 33.18%.
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등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Aakre, C.D. / 2013 / A bacterial toxin inhibits DNA replication elongation through a direct interaction with the beta sliding clamp / Mol Cell 52:617~628
Aizenman, E. / 1996 / An Escherichia coli chromosomal "addiction module" regulated by guanosine [corrected] 3',5'-bispyrophosphate: a model for programmed bacterial cell death / Proc Natl Acad Sci U S A 93:6059~6063
Battista, J.R. / 1999 / Why is Deinococcus radiodurans so resistant to ionizing radiation? / Trends Microbiol 7:362~365
Daly, M.J. / 2009 / A new perspective on radiation resistance based on Deinococcus radiodurans / Nat Rev Microbiol 7:237~245
Emsley, P. / 2004 / Coot: model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Aizenman, E. / 1996 / An Escherichia coli chromosomal "addiction module" regulated by guanosine [corrected] 3',5'-bispyrophosphate: a model for programmed bacterial cell death / Proc Natl Acad Sci U S A 93:6059~6063
Battista, J.R. / 1999 / Why is Deinococcus radiodurans so resistant to ionizing radiation? / Trends Microbiol 7:362~365
Daly, M.J. / 2009 / A new perspective on radiation resistance based on Deinococcus radiodurans / Nat Rev Microbiol 7:237~245
Emsley, P. / 2004 / Coot: model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
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