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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Many kinds of nucleotides are differentially synthesized and degraded in response to external and internal signaling inbacterial cells. Cyclic di-GMP (c-di-GMP) is ubiquitous bacterial nucleotide secondary signaling molecule, and is involvedin virulence, antibiotic resistance, biofilm formation and cell division. VCA0593 was predicted or screened as a c-di-GMPor 5’-phosphguanylyl-(3’,5’)-guanosine binding protein in Vibrio cholerae genome by genome-wide system approaches,suggesting that VCA0593 might be involved in a c-di-GMP-mediated signaling pathway. In this study, the full-lengthVCA0593 was overexpressed in the E. coli expression system, and successfully crystallized. X-rays were diffracted by thecrystals to 1.60 A resolution, revealing that the crystals belong to space group P21212, with unit cell parameters a = 68.9, b= 149.0, and c = 58.9 A. According to cell content analysis, the asymmetric unit was expected to contain two molecules ofVCA0593. The molecular replacement was not available due to lack of the homologous models. We are now under way tosolve the crystal structure using the anomalous signals from Zn-soaked crystals. The crystal structure would help revealthe function of the protein and its mechanism of action, which could be associated with the pathogenesis of V. cholerae.
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참고문헌 (11)
참고문헌 신청
Beyhan, S / 2007 / Smooth to rugose phase variation in Vibrio cholerae can be mediated by a single nucleotide change that targets c-di-GMP signalling pathway / Mol Microbiol 63:995~1007
Cohen, D / 2015 / Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa / Proc Natl Acad Sci USA 112:11359~11364
Conner, J. G / 2017 / The ins and outs of cyclic di-GMP signaling in Vibrio cholerae / Curr Opin Microbiol 36:20~29
Corrigan, R. M / 2011 / c-di-AMP is a new second messenger in Staphylococcus aureus with a role in controlling cell size and envelope stress / PLoS Pathog 7:e1002217
D'Argenio, D. A / 2004 / Cyclic di-GMP as a bacterial second messenger / Microbiol 150:2497~2502
Cohen, D / 2015 / Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa / Proc Natl Acad Sci USA 112:11359~11364
Conner, J. G / 2017 / The ins and outs of cyclic di-GMP signaling in Vibrio cholerae / Curr Opin Microbiol 36:20~29
Corrigan, R. M / 2011 / c-di-AMP is a new second messenger in Staphylococcus aureus with a role in controlling cell size and envelope stress / PLoS Pathog 7:e1002217
D'Argenio, D. A / 2004 / Cyclic di-GMP as a bacterial second messenger / Microbiol 150:2497~2502
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