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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제2호
- 발행연도
- 2019.1
- 수록면
- 274 - 282 (9page)
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Mercury-resistant (HgR) bacteria were isolated from heavy metal polluted wastewater and soil collected near to tanneries of district Kasur, Pakistan. Bacterial isolates AZ-1, AZ-2 and AZ-3 showed resistance up to 40 μg/ml against mercuric chloride (HgCl2). 16S rDNA ribotyping and phylogenetic analysis were performed for the characterization of selected isolates as Bacillus sp. AZ-1 (KT270477), Bacillus cereus AZ-2 (KT270478) and Bacillus cereus AZ-3 (KT270479). Phylogenetic relationship on the basis of merA nucleotide sequence confirmed 51- 100% homology with the corresponding region of the merA gene of already reported mercuryresistant Gram-positive bacteria. The merE gene involved in the transportation of elemental mercury (Hg0) via cell membrane was cloned for the first time into pHLV vector and transformed in overexpressed C43(DE3) E. coli cells. The recombinant plasmid (pHLMerE) was expressed and the native MerE protein was obtained after thrombin cleavage by size exclusion chromatography (SEC). The purification of fusion/recombinant and native protein MerE by Ni-NTA column, dialysis and fast protein liquid chromatography (FPLC/SEC) involved unfolding/refolding techniques. A small-scale reservoir of wastewater containing 30 μg/ml of HgCl2 was designed to check the detoxification ability of selected strains. It resulted in 83% detoxification of mercury by B. cereus AZ-2 and B. cereus AZ-3, and 76% detoxification by Bacillus sp. AZ-1 respectively (p < 0.05).
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참고문헌 (42)
참고문헌 신청
Steenhuisen F / 2015 / Identifying and characterizing major emission point sources as a basis for geospatial distribution of mercury emissions inventories / Atmos. Environ 112:167~177
Santos-Gandelman JF / 2014 / Mercury and methylmercury detoxification potential by sponge-associated bacteria / Antonie Leeuwenhoek 106:585~590
Abbas Z / 2012 / Toxicity of mercury in different samples(water and soils)and its exposure in pakistan / Sci. Int 24:421~429
Sedlmeier R / 1992 / Cloning and DNA sequence analysis of the mercury resistance genes of Streptomyces lividans / Mol. Gen. Genet 236:76~85
Nakamura K / 1994 / Molecular analysis of mercuryresistant Bacillus isolates from sediment of Minamata Bay, Japan / Appl. Environ. Microbiol 60:4596~4599
Santos-Gandelman JF / 2014 / Mercury and methylmercury detoxification potential by sponge-associated bacteria / Antonie Leeuwenhoek 106:585~590
Abbas Z / 2012 / Toxicity of mercury in different samples(water and soils)and its exposure in pakistan / Sci. Int 24:421~429
Sedlmeier R / 1992 / Cloning and DNA sequence analysis of the mercury resistance genes of Streptomyces lividans / Mol. Gen. Genet 236:76~85
Nakamura K / 1994 / Molecular analysis of mercuryresistant Bacillus isolates from sediment of Minamata Bay, Japan / Appl. Environ. Microbiol 60:4596~4599
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