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논문 기본 정보

자료유형
학술저널
저자정보
Cheng Jie (College of Agronomy Northwest A&F University) Wei Fan (Northwest A & F University) Zhang Mingfei (Chi Feng University) Li Nan (Chi Feng University) Song Tianqi (College of Agronomy Northwest A&F University) Wang Yong (Northwest A & F University) Chen Dongsheng (The Crop Research Institute Ningxia Academy of Agriculture and Forestry Science) Xiang Jishan (Chi Feng University) Zhang Xiaoke (College of Agronomy Northwest A&F University)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.9
발행연도
2021.9
수록면
1,035 - 1,048 (14page)
DOI
10.1007/s13258-021-01115-x

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Background Cloning and characterizing the drought-inducible promoters is essential for their use in crop resistance’s genetic improvement. Previous studies have shown that the TaNRX1-D gene participates in regulating the response of wheat to drought stress. However, its promoter has not yet been identifed. Objective In this study, we aimed to characterize the promoter of the TaNRX1-D gene. Methods The promoter of TaNRX1-D (named P0, 2081 bp) was isolated from common wheat with several cis-acting elements that regulate in response to abiotic stresses and some core cis-acting elements. Functional verifcation of the promoter, eight 5′-deletion fragments of TaNRX1-D promoter, was fused to the β-glucuronidase (GUS) gene P0::GUS~P7::GUS and transformed into Arabidopsis, respectively. Agrobacterium-mediated GUS transient assay the P6a and P6b promoter regions in tobacco leaves under normal, osmotic or ABA stress. Results Activity analysis of the full-length promoter (P0) showed that the intensity of stronger β-glucuronidase (GUS) staining in the roots and leaves was obtained during the growth of transgenic Arabidopsis. P0::GUS displayed the GUS activity was much higher in the roots and leaves than in other parts of the transgenic plant under normal conditions, which was similarly within wheat. Analysis of the 5′-deletion fragments revealed that P0::GUS~P6::GUS responded well upon exposure to osmotic (polyethylene glycol-6000, PEG6000) and abscisic acid (ABA) stress treatments and expressed signifcantly higher GUS activity than the CaMV35S promoter (35S::GUS), while P7::GUS did not. GUS transient assay in tobacco leaves showed that the GUS activities of P6a and P6b were lower than P6 in the PEG6000 and ABA stresses. Conclusion The 193 bp (P6) segment was considered the core region of TaNRX1-D responding to PEG6000 or ABA treatment. GUS activity assay in transgenic Arabidopsis showed that this segment was sufcient for the PEG6000 or ABA stress response. The identifed 193 bp promoter of TaNRX1-D in this study will help breed osmotic or ABA tolerant crops. The 36 bp segment between P6 and P6b (?193 to ?157 bp) was considered the critical sequence for the TaNRX1-D gene responding to PEG6000 or ABA treatment.
#Common wheat #TaNRX1-D promoter #Osmotic or ABA stress #Cis-regulatory element

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참고문헌 (47)

참고문헌 신청
Dalia Z. Alomari / 2018 / Identifying Candidate Genes for Enhancing Grain Zn Concentration in Wheat / Frontiers in Plant Science 9:1313~1324 Crossref Elias S. J. Arnér / 2000 / Physiological functions of thioredoxin and thioredoxin reductase / European Journal of Biochemistry 267(20):6102~6109 Crossref Papri Basak / 2018 / Functional characterization of two myo-inositol-1-phosphate synthase (MIPS) gene promoters from the halophytic wild rice (Porteresia coarctata) / Planta 248(5):1121~1141 Crossref Saadia Bihmidine / 2013 / Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress / Planta 237(1):55~64 Crossref Alan H. Christensen / 1992 / Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation / Plant Molecular Biology 18(4):675~689 Crossref

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