지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 337 - 342 (6page)
이용수
초록· 키워드
오류제보하기
We published that bacterial heme was over-produced in a recombinant Corynebacterium glutamicum expressing 5-aminolevulinic acid synthase (hemA+) under control of a constitutive promoter (P180) and the heme-producing C. glutamicum had commercial potentials; as an iron feed additive for swine and as a preservative for lactic acid bacteria. To enhance the heme production, the hemA+ gene was expressed under controls of various promoters in the recombinant C. glutamicum. The hemA+ expression by PgapA (a constitutive glycolytic promoter of glyceraldehyde-3-phosphate dehydrogenase) led 75% increase of heme production while the expression by PH36 (a constitutive, very strong synthetic promoter) resulted in 50% decrease compared with the control (hemA+ expression by P180 constitutive promoter). The hemA+ expression by a late log-phase activating Psod (an oxidative-stress responding promoter of superoxide dismutase) led 50% greater heme production than the control. The hemA+ expression led by a heat-shock responding chaperone promoter (PdnaK) resulted in 121% increase of heme production at the optimized heat-shock conditions. The promoter stren
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Becker J / 2007 / Metabolic flux engineering of l-lysine production in Corynebacterium glutamicum—over expression and modification of G6P dehydrogenase / J. Biotechnol 132:99~109
Kim HJ / 2012 / An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production / Appl. Microbiol. Biotechnol 94:1079~1086
Schobert M / 2002 / Regulation of heme biosynthesis in non-phototrophic bacteria / J. Mol. Microbiol. Biotechnol 4:287~294
Verkamp E / 1989 / Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene / J. Bacteriol 171:4728~4735
McNicholas PM / 1997 / Expression of the heme biosynthetic pathway genes hemCD, hemH, hemM and hemA of Escherichia coli / FEMS Microbiol. Lett 146:143~148
Kim HJ / 2012 / An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production / Appl. Microbiol. Biotechnol 94:1079~1086
Schobert M / 2002 / Regulation of heme biosynthesis in non-phototrophic bacteria / J. Mol. Microbiol. Biotechnol 4:287~294
Verkamp E / 1989 / Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene / J. Bacteriol 171:4728~4735
McNicholas PM / 1997 / Expression of the heme biosynthetic pathway genes hemCD, hemH, hemM and hemA of Escherichia coli / FEMS Microbiol. Lett 146:143~148
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Heme Derived from Corynebacterium glutamicum: A Potential Iron Additive for Swine and an Electron Carrier Additive for Lactic Acid Bacterial Culture
Journal of Microbiology and Biotechnology
2017 .01
-
Development of Synthetic Promoters and Signal Peptides in Corynebacterium glutamicum for Efficient Secretory Production of Recombinant Proteins
한국미생물학회 학술대회논문집
2015 .04
-
Building the Frequency Profile of the Core Promoter Element Patterns in the Three ChromHMM Promoter States at 200bp Intervals: A Statistical Perspective
Genomics & informatics
2015 .01
-
Identification and Validation of Four Novel Promoters for Gene Engineering with Broad Suitability across Species
Journal of Microbiology and Biotechnology
2021 .08
-
Deinococcus radiodurans 유래 DR1558과 PprM에 의한 Corynebacterium glutamicum의 라이신 생산 향상 연구
한국미생물·생명공학회지
2017 .01
-
Analysis of Heme Biosynthetic Pathways in a Recombinant Escherichia coli
Journal of Microbiology and Biotechnology
2015 .01
-
Increase of a Fibrinolytic Enzyme Production through Promoter Replacement of aprE3-5 from Bacillus subtilis CH3-5
Journal of Microbiology and Biotechnology
2021 .01
-
Core Promoter Mutation of ntC1731T and G1806A of Hepatitis B Virus Increases HBV Gene Expression
생명과학회지
2022 .02
-
Optimization of a Fermentation Process to Improve Microbial Production of Heme
한국미생물학회 학술대회논문집
2022 .10
-
Engineering of Corynebacterium glutamicum for the Secretory Production of Recombinant Proteins
한국미생물학회 학술대회논문집
2018 .04
-
Cell-free Systems for RNA Detection and Promoter Prototyping
한국미생물학회 학술대회논문집
2021 .08
-
Heterologous Gene Expression System Using the Cold-Inducible CnAFP Promoter in Chlamydomonas reinhardtii
Journal of Microbiology and Biotechnology
2020 .01
-
The Association between Maternal Dietary Iron Intake during the First Trimester of Pregnancy with Pregnancy Outcomes and Pregnancy-Related Complications
Clinical Nutrition Research
2020 .01
-
Substitution of the Native Promoter of an rRNA Operon in Escherichia coli to an rRNA Promoter of Vibrio natriegens for Accelerate Growth
한국미생물학회 학술대회논문집
2017 .04
-
Metabolic Engineering for High-level L-Arginine Production in Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2019 .04
-
Metabolic Engineering of Corynebacterium glutamicum for High-Level L-Arginine Production
한국미생물학회 학술대회논문집
2015 .04
-
The Actinobacterium Corynebacterium glutamicum, an Industrial Workhorse
Journal of Microbiology and Biotechnology
2016 .01
-
Effect of Heme-rich Nutrient on Anaerobic Bacterial Growth and Survival: A Model Study on Lactobacillus gasseri
한국미생물·생명공학회지
2021 .01
-
Metabolic Engineering of Escherichia coli for Secretory Production of Free Heme
한국미생물학회 학술대회논문집
2019 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0