지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.8
- 수록면
- 577 - 586 (10page)
이용수
초록· 키워드
오류제보하기
Phytocystatins (PhyCYSs) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. Here, we characterized a PhyCYS from Arabidopsis thaliana, which was designated AtCYS5. RT-qPCR analysis showed that the expression of AtCYS5 in germinating seeds was induced by heat stress (HS) and exogenous abscisic acid (ABA) treatment. Analysis of the expression of the β-glucuronidase reporter gene under the control of the AtCYS5 promoter showed that AtCYS5 expression during seed germination was induced by HS and ABA. Constitutive overexpression of AtCYS5 driven by the cauliflower mosaic virus 35S promoter led to enhanced HS tolerance in transgenic Arabidopsis, which was characterized by higher fresh weight and root length compared to wild-type (WT) and knockout (cys5) plants grown under HS conditions. The HS tolerance of AtCYS5-overexpressing transgenic plants was associated with increased insensitivity to exogenous ABA during both seed germination and post-germination compared to WT and cys5. Although no HS elements were identified in the 5’-flanking region of AtCYS5, canonical ABA-responsive elements (ABREs) were detected. AtCYS5 was upregulated in ABA-treated protoplasts transiently co-expressing this gene and genes encoding bZIP ABRE-binding factors (ABFs and AREB3). In the absence of ABA, ABF1 and ABF3 directly bound to the ABREs in the AtCYS5 promoter, which activated the transcription of this gene in the presence of ABA. These results suggest that an ABA-dependent pathway plays a positive role in the HS-responsive expression of AtCYS5 during seed germination and post-germination growth.
목차
등록된 정보가 없습니다.
참고문헌 (35)
참고문헌 신청
Bae, E. K. / 2010 / Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis / Plant Physiol. Biochem 48:136~141
Chen, H. / 2010 / Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response / Biochem. Biophys. Res. Commun 401:238~244
Christoff, A. P. / 2016 / Rice bifunctional phytocystatin is a dual molulator of legumain and papain-like proteases / Plant Mol. Biol 92:193~207
Clough, S. J. / 1998 / Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana / Plant J 16:735~743
Fujita, Y. / 2005 / AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis / Plant Cell 17:3470~3488
Chen, H. / 2010 / Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response / Biochem. Biophys. Res. Commun 401:238~244
Christoff, A. P. / 2016 / Rice bifunctional phytocystatin is a dual molulator of legumain and papain-like proteases / Plant Mol. Biol 92:193~207
Clough, S. J. / 1998 / Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana / Plant J 16:735~743
Fujita, Y. / 2005 / AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis / Plant Cell 17:3470~3488
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling
Molecules and Cells
2016 .02
-
Arabidopsis MAP3K16 and Other Salt-Inducible MAP3Ks Regulate ABA Response Redundantly
Molecules and Cells
2017 .03
-
Identification of a 193 bp promoter region of TaNRX1?D gene from common wheat that contributes to osmotic or ABA stress inducibility in transgenic Arabidopsis
Genes & Genomics
2021 .09
-
배초향 종자처리가 발아성 향상에 미치는 영향
한국환경과학회지
2021 .01
-
수박종자의 Osmotic Priming 처리가 종자활력 및 유묘생장에 미치는 영향
한국환경과학회지
2016 .04
-
RAV1 Negatively Regulates Seed Development by Directly Repressing MINI3 and IKU2 in Arabidopsis
Molecules and Cells
2018 .12
-
Arabidopsis Raf-Like Kinase Raf10 Is a Regulatory Component of Core ABA Signaling
Molecules and Cells
2019 .01
-
식물의 물부족 스트레스 신호 전달 네트워크에 대한 이해
생명과학회지
2018 .03
-
The phytohormone abscisic acid increases triacylglycerol content in the green microalga Chlorella saccharophila (Chlorophyta)
Algae
2016 .01
-
Analysis of reactive species in water activated by plasma and application to seed germination
한국진공학회 학술발표회초록집
2015 .08
-
염화물계 제설제의 농도처리에 따른 벼과와 국화과의 종자 발아특성
한국환경과학회지
2019 .11
-
Gene expression profiling for seed protein and oil synthesis during early seed development in soybean
Genes & Genomics
2015 .01
-
The Shaker Type Potassium Channel, GORK, Regulates Abscisic Acid Signaling in Arabidopsis
The Plant Pathology Journal
2019 .12
-
Induced Tolerance to Salinity Stress by Halotolerant Bacteria Bacillus aryabhattai H19-1 and B. mesonae H20-5 in Tomato Plants
Journal of Microbiology and Biotechnology
2019 .01
-
Conserved co-functional network between maize and Arabidopsis aid in the identification of seed defective genes in maize
Genes & Genomics
2021 .01
-
Effects of Salt Treatment on Seed Germination and Plant Growth of Korean Native Apocynum lancifolium Russanov
한국환경과학회지
2021 .11
-
The SnRK2 family in pepper (Capsicum annuum L.): genome-wide identifcation and expression analyses during fruit development and under abiotic stress
Genes & Genomics
2020 .01
-
Genome-wide identification, characterisation and expression profile analysis of DEAD-box family genes in sweet potato wild ancestor Ipomoea trifida under abiotic stresses
Genes & Genomics
2020 .01
-
PLASMA ASSISTED NITROGEN FIXATION FOR THE ELEVATION OF SEED GERMINATION AND PLANT GROWTH
한국진공학회 학술발표회초록집
2020 .02
-
Heterologous Expression of the Hot Pepper ABA 8’-Hydroxylase in Escherichia coli for Phaseic Acid Production
Journal of Microbiology and Biotechnology
2023 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0