지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 254 - 261 (8page)
이용수
초록· 키워드
오류제보하기
Glioblastomas (GBM) are very difficult to treat and their aggressiveness is one of the main reasons for this as well as for the frequent recurrences. MicroRNAs post-transcriptionally regulate their target genes through interaction between their seed sequence and 3?UTR of the target mRNAs. We previously reported that miR-296-3p is regulated by neurofibromatosis 2 (NF2) and enhances the invasiveness of GBM cells via SOCS2/STAT3. In this study, we investigated whether miR-296-5p, which originates from the same precursor miRNA as miR-296-3p, can increase the invasiveness of GBM cells. It was observed that miR-296-5p potentiated the invasion of various GBM cells including LN229, T98G, and U87MG. Through bioinformatics approaches, two genes were identified as miR-296-5p targets: caspase-8 (CASP8) and nerve growth factor receptor (NGFR). From results obtained from Ago2 immunoprecipitation and luciferase assays, we found that miR-296-5p downregulates CASP8 and NGFR through direct interaction between seed sequence of the miRNA and 3?UTR of the target mRNA. Knockdown of CASP8 or NGFR also increased the invasive ability of GBM cells, indi-cating that CASP8 and NGFR are involved in potentiation of invasiveness by miR-296-5p. Consistent with our findings, CASP8 was downregulated in brain metastatic lung cancer cells, which have a high level of miR-296-5p, compared to parental cells, suggesting that miR-296-5p may be generally associated with the acquisition of invasiveness. Collectively, our results implicate miR-296-5p as a potential cause of invasiveness in cancer and suggest it as a promising ther-apeutic target for GBM.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Barker, P. A. / 2004 / p75NTR is positively promiscuous : novel partners and new insights / Neuron 42:529~533
Freije, W. A. / 2004 / Gene expression profiling of gliomas strongly predicts survival / Cancer Res 64:6503~6510
Hopkins-Donaldson, S. / 2003 / Silencing of death receptor and caspase-8 expression in small cell lung carcinoma cell lines and tumors by DNA methylation / Cell Death Differ 10:356~364
Hwang, S. J. / 2015 / Overexpression of microRNA-95-3p suppresses brain metastasis of lung adenocarcinoma through downregulation of cyclin D1 / Oncotarget 6:20434~20448
Hwang, S. J. / 2016 / Ubiquitin-specific protease 4 controls metastatic potential through beta-catenin stabilization in brain metastatic lung adenocarcinoma / Sci. Rep 6:21596
Freije, W. A. / 2004 / Gene expression profiling of gliomas strongly predicts survival / Cancer Res 64:6503~6510
Hopkins-Donaldson, S. / 2003 / Silencing of death receptor and caspase-8 expression in small cell lung carcinoma cell lines and tumors by DNA methylation / Cell Death Differ 10:356~364
Hwang, S. J. / 2015 / Overexpression of microRNA-95-3p suppresses brain metastasis of lung adenocarcinoma through downregulation of cyclin D1 / Oncotarget 6:20434~20448
Hwang, S. J. / 2016 / Ubiquitin-specific protease 4 controls metastatic potential through beta-catenin stabilization in brain metastatic lung adenocarcinoma / Sci. Rep 6:21596
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
The seed sequence is necessary but insufficient for downregulation of target genes by miR-608
Genes & Genomics
2016 .01
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
-
miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs
Molecules and Cells
2016 .04
-
MicroRNA-203 As a Stemness Inhibitor of Glioblastoma Stem Cells
Molecules and Cells
2016 .08
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
-
MiR-1299 functions as a tumor suppressor to inhibit the proliferation and metastasis of gastric cancer by targeting ARF6
Genes & Genomics
2022 .02
-
Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer
대한임상검사과학회지
2017 .01
-
Overexpression of Long Non-Coding RNA MIR22HG Represses Proliferation and Enhances Apoptosis via miR-629-5p/TET3 Axis in Osteosarcoma Cells
Journal of Microbiology and Biotechnology
2021 .10
-
LncRNA H19 Drives Proliferation of Cardiac Fibroblasts and Collagen Production via Suppression of the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β Axis
Molecules and Cells
2022 .03
-
miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro
Molecules and Cells
2018 .03
-
High throughput sequencing of herbaceous peony small RNAs to screen thermo-tolerance related microRNAs
Genes & Genomics
2017 .01
-
Suppression of HELLS by miR-451a represses mTOR pathway to hinder aggressiveness of SCLC
Genes & Genomics
2021 .01
-
Hsa_Circ_0001947/MiR-661/DOK7 Axis Restrains Non-Small Cell Lung Cancer Development
Journal of Microbiology and Biotechnology
2021 .11
-
The MicroRNA-551a/MEF2C Axis Regulates the Survival and Sphere Formation of Cancer Cells in Response to 5-Fluorouracil
Molecules and Cells
2019 .01
-
The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells
Journal of Microbiology and Biotechnology
2021 .01
-
Alteration of the Metabolome Profile in Endothelial Cells by Overexpression of miR-143/145
Journal of Microbiology and Biotechnology
2016 .01
-
MicroRNA?9 inhibits proliferation and progression in retinoblastoma cells by targeting PTEN
Genes & Genomics
2021 .09
-
MiR-200c-3p inhibits LPS-induced M1 polarization of BV2 cells by targeting RIP2
Genes & Genomics
2022 .04
-
Roles of miR-128 in Myogenic Differentiation and Insulin Signaling in Rat L6 Myoblasts
생명과학회지
2020 .09
-
Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
Molecules and Cells
2018 .02
이 논문의 저자 정보
최근 본 자료
댓글(0)
0