지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
HK shiitake mushroom mycelium (HKSMM: 14% β-glucan)은 식품의약품안전처의 개별인정형 간 건강기능식품 원료이다. HKSMM의 50% ethanol 추출물 (HKSMM50)에 대한 면역증강 효과를 Concanavalin A(ConA)로 활성화한 human T lymphocyte Jurkat cells에서 연구하였다. Active hexose correlated compound (AHCC)는 positive control로 사용하였다. ConA로 활성화한 Jurkat cells에 HKSMM50 (0, 25, 50, 100 μg g/ml) 및 AHCC (100 μg g/ml)를 처리하고 3시간 또는 6시간 배양하였다. Jurkat cells의 cytosol과 nucleus에 함유된 transcription factor인 nuclear factor of activated T cells (NFAT) 함량은 Western blotting으로 측정하였다. Interleukin-2 (IL-2)와 interferon-gamma (IFN-γ) 함량 및 cyclooxygenase-2 (COX-2) 활성은 enzyme-linked immunosorbent assay (ELISA) kit로 분석하였다. HKSMM50은 cytosolic NFAT protein 함량은 낮추었고, nuclear NFAT protein함량은 증가시켰다. IL-2와 IFN-γ 함량은 증가되었고, COX-2 활성과 apoptosis는 억제되었다. AHCC 효과는 HKSSM50의 효과와 유사하였다. 이와 같은 결과는 HKSMM50가 ConA로 활성화된 Jurkat cells에서 NFAT protein을 활성화시켜, IL-2와 IFN-γ 함량을 증가시켰음을 의미한다. 또한 HKSMM50은 COX-2 활성과 apoptosis를 억제하였다. 따라서 이와 같은 결과는 HKSMM이 면역증진을 위한 건강기능식품 원료로 사용할 수 있음을 의미한다.
목차
서론
재료 및 방법
결과
고찰
References
초록
참고문헌 (47)
참고문헌 신청
Adan, A., Kiraz, Y. and Yusuf, B. 2016. Cell proliferation and cytotoxicity assays. Curr. Pharm. Biotech. 17, 1213-1221.
Al-Daghri, N. M., Alokail, M. S., Alkharfy, K. M., Mohammed, A. K., Abd-Alrahman, S. H., Yakout, S. M., Amer, O. E. and Krishnaswamy, S. 2012. Fenugreek extract as an inducer of cellular death via autophagy in human T lymphoma Jurkat cells. BMC Complement. Altern. Med. 12, 202.
Aleem, E. 2013. β-Glucans and their applications in cancer therapy: focus on human studies. Anticancer Agents Med. Chem. 13, 709-719.
Bisen, P. S., Baghe, R. K., Sanodiya, B. S., Thakur, G. S. and Prasad, G. B. K. S. 2010. Lentinus edodes: A macrofungus with pharmacological activities. Cur. Med. Chem. 17, 2419-2430.
Chan, G. C. F., Chan, W. K. and Sze, D. M. Y. 2009. The effects of β-glucan on human immune and cancer cells. J. Hematol. Oncol. 2, 25-36.
Al-Daghri, N. M., Alokail, M. S., Alkharfy, K. M., Mohammed, A. K., Abd-Alrahman, S. H., Yakout, S. M., Amer, O. E. and Krishnaswamy, S. 2012. Fenugreek extract as an inducer of cellular death via autophagy in human T lymphoma Jurkat cells. BMC Complement. Altern. Med. 12, 202.
Aleem, E. 2013. β-Glucans and their applications in cancer therapy: focus on human studies. Anticancer Agents Med. Chem. 13, 709-719.
Bisen, P. S., Baghe, R. K., Sanodiya, B. S., Thakur, G. S. and Prasad, G. B. K. S. 2010. Lentinus edodes: A macrofungus with pharmacological activities. Cur. Med. Chem. 17, 2419-2430.
Chan, G. C. F., Chan, W. K. and Sze, D. M. Y. 2009. The effects of β-glucan on human immune and cancer cells. J. Hematol. Oncol. 2, 25-36.
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UCI(KEPA) : I410-ECN-0101-2023-470-000129064