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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 294 - 305 (12page)
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초록· 키워드
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The application of decellularized extracellular matrices to aid tissue regeneration in reconstructive surgery and regenerative medicine has been promising. Several decellularization protocols for removing cellular materials from natural tissues such as heart valves are currently in use. This paper evaluates the feasibility of potential extension of this methodology relative to the desirable properties of load bearing joint tissues such as stiffness, porosity and ability to recover adequately after deformation to facilitate physiological function. Two decellularization protocols, namely: Trypsin and Triton X-100 were evaluated against their effects on bovine articular cartilage, using biomechanical, biochemical and microstructural techniques. These analyses revealed that decellularization with trypsin resulted in severe loss of mechanical stiffness including deleterious collapse of the collagen architecture which in turn significantly compromised the porosity of the construct. In contrast, triton X-100 detergent treatment yielded samples that retain mechanical stiffness relative to that of the normal intact cartilage sample, but the resulting construct contained ruminant cellular constituents. We conclude that both of these common decellularization protocols are inadequate for producing constructs that can serve as effective replacement and scaffolds to regenerate articular joint tissue.
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참고문헌 (47)
참고문헌 신청
Metcalf MH / 2002 / Surgical technique for xenograft(SIS)augmentation of rotator-cuff repairs / Oper Tech Orthop 12:204~208
Hung KC / 2014 / Synthesis and 3D printing of biodegradable polyurethane elastomer by a water-based process for cartilage tissue engineering applications / Adv Healthc Mater 3:1578~1587
Badylak SF / 2007 / The extracellular matrix as a biologic scaffold material / Biomaterials 28:3587~3593
Ren H / 2013 / Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold / Liver Int 33:448~458
Oliveira AC / 2013 / Evaluation of small intestine grafts decellularization methods for corneal tissue engineering / PLoS One 8:e66538
Hung KC / 2014 / Synthesis and 3D printing of biodegradable polyurethane elastomer by a water-based process for cartilage tissue engineering applications / Adv Healthc Mater 3:1578~1587
Badylak SF / 2007 / The extracellular matrix as a biologic scaffold material / Biomaterials 28:3587~3593
Ren H / 2013 / Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold / Liver Int 33:448~458
Oliveira AC / 2013 / Evaluation of small intestine grafts decellularization methods for corneal tissue engineering / PLoS One 8:e66538
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