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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 433 - 443 (11page)
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초록· 키워드
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Background: Respiratory mucosa defects result in airway obstruction and infection, requiring subsequent functional recovery of the respiratory epithelium. Because site-specific extracellular matrix (ECM) facilitates restoration of organ function by promoting cellular migration and engraftment, previous studies considered decellularized trachea an ideal ECM; however, incomplete cell removal from cartilage and mucosal-architecture destruction are frequently reported. Here, we developed a decellularization protocol and applied it to the respiratory mucosa of separated porcine tracheas. Methods: The trachea was divided into groups according to decellularization protocol: native mucosa, freezing–thawing (FT), FT followed by the use of Perasafe-based chemical agents before mucosal separation (wFTP), after mucosal separation (mFTP), and followed by DNase decellularization (mFTD). Decellularization efficacy was evaluated by DNA quantification and hematoxylin and eosin staining, and ECM content of the scaffold was evaluated by histologic analysis and glycosaminoglycan and collagen assays. Biocompatibility was assessed by cell-viability assay and in vivo transplantation. Results: The mFTP mucosa showed low antigenicity and maintained the ECM to form a proper microstructure. Additionally, tonsil-derived stem cells remained viable when cultured with or seeded onto mFTP mucosa, and the in vivo host response showed a constructive pattern following implantation of the mFTP scaffolds. Conclusion: These results demonstrated that xenogenic acellular respiratory mucosa matrix displayed suitable biocompatibility as a scaffold material for respiratory mucosa engineering.
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참고문헌 (34)
참고문헌 신청
Tsukada H / 2004 / Experimental study of a new tracheal prosthesis : pored Dacron tube / J Thorac Cardiovasc Surg 127:877~884
김한수 / 2011 / Development of an Artificial Tracheal Prosthesis: a Semicircular Shape Polyurethane Scaffold / 조직공학과 재생의학 8(5):439~445
Chang JW / 2014 / Tissue-engineered tracheal reconstruction using three-dimensionally printed artificial tracheal graft : preliminary report / Artif Organs 38:E95~E105
Hong HJ / 2014 / Tracheal reconstruction using chondrocytes seeded on a poly(Llactic-co-glycolic acid)-fibrin/hyaluronan / J Biomed Mater Res A 102:4142~4150
Lee JH / 2014 / Triplelayered polyurethane prosthesis with wrinkles for repairing partial tracheal defects / Laryngoscope 124:2757~2763
김한수 / 2011 / Development of an Artificial Tracheal Prosthesis: a Semicircular Shape Polyurethane Scaffold / 조직공학과 재생의학 8(5):439~445
Chang JW / 2014 / Tissue-engineered tracheal reconstruction using three-dimensionally printed artificial tracheal graft : preliminary report / Artif Organs 38:E95~E105
Hong HJ / 2014 / Tracheal reconstruction using chondrocytes seeded on a poly(Llactic-co-glycolic acid)-fibrin/hyaluronan / J Biomed Mater Res A 102:4142~4150
Lee JH / 2014 / Triplelayered polyurethane prosthesis with wrinkles for repairing partial tracheal defects / Laryngoscope 124:2757~2763
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