지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 369 - 376 (8page)
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초록· 키워드
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BACKGROUND: Tissue decellularization has evolved as a promising approach for tissue engineering applications. METHODS: In this study, we harvested fascial tissue from porcine anterior abdominal wall and the samples were decellularized with a combination of agents such as Triton X-100, trypsin and DNAase. Afterwards, we evaluated cell removal by histological analysis and DNA quantification. Mechanical functionality was evaluated by applying a range of hydrostatic pressures. A sample of decellularized fascia was transplanted into a rabbit and after 15 days a biopsy of this tissue was examined; the animal was observed during 6 months after surgery. RESULTS: The extracellular matrix was retained with a complete decellularization as evidenced by histologic examination. The DNA content was significantly reduced. The scaffold preserved its tensile mechanical properties. The graft was incorporated into a full thickness defect made in the rabbit abdominal wall. This tissue was infiltrated by granulation and inflammatory cells and the histologic structure was preserved 15 days after surgery. The animal did not develop hernias, infections or other complications, after a 6-months of follow up. CONCLUSIONS: The protocol of decellularization of fascial tissue employed in this study proved to be efficient. The mechanical test demonstrated that the samples were not damaged and maintained its physical characteristics; clinical evolution of the rabbit, recipient of the decellularized fascia, demonstrated that the graft was effective as a replacement of native tissue.In conclusion, a biological scaffold derived from porcine fascial tissue may be a suitable candidate for tissue engineering applications.
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참고문헌 (30)
참고문헌 신청
Fink C / 2014 / Incisional hernia rate 3 years after midline laparotomy / Br J Surg 101:51~54
Liasis L / 2013 / Traumatic abdominal wall hernia: is the treatment strategy a real problem? / J Trauma Acute Care Surg 74:1156~1162
Rosen MJ / 2013 / A 5-year clinical experience with single-staged repairs of infected and contaminated abdominal wall defects utilizing biologic mesh / Ann Surg 257:991~996
Risby K / 2016 / Congenital abdominal wall defects : staged closure by Dual Mesh / J Neonatal Surg 5:2
Ohira G / 2015 / Synthetic polyglycomer short-term absorbable sutures versus polydioxanone long-term absorbable sutures for preventing incisional hernia and wound dehiscence after abdominal wall closure: a comparative randomized study of patients treated for gastric or colon cancer / Surg Today 45:841~845
Liasis L / 2013 / Traumatic abdominal wall hernia: is the treatment strategy a real problem? / J Trauma Acute Care Surg 74:1156~1162
Rosen MJ / 2013 / A 5-year clinical experience with single-staged repairs of infected and contaminated abdominal wall defects utilizing biologic mesh / Ann Surg 257:991~996
Risby K / 2016 / Congenital abdominal wall defects : staged closure by Dual Mesh / J Neonatal Surg 5:2
Ohira G / 2015 / Synthetic polyglycomer short-term absorbable sutures versus polydioxanone long-term absorbable sutures for preventing incisional hernia and wound dehiscence after abdominal wall closure: a comparative randomized study of patients treated for gastric or colon cancer / Surg Today 45:841~845
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