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논문 기본 정보

자료유형
학술저널
저자정보
Saifun Nahar (Graduate School of Medicine University of the Ryukyus) Akira Hokama (Graduate School of Medicine University of the Ryukyus) Atsushi Iraha (Graduate School of Medicine University of the Ryukyus) Tetsuya Ohira (Graduate School of Medicine University of the Ryukyus) Tetsu Kinjo (Graduate School of Medicine University of the Ryukyus) Tetsuo Hirata (Graduate School of Medicine University of the Ryukyus) Takeshi Kinjo (Graduate School of Medicine University of the Ryukyus) Gretchen L. Parrott (Graduate School of Medicine University of the Ryukyus) Jiro Fujita (Graduate School of Medicine University of the Ryukyus)
저널정보
대한장연구학회 Intestinal research Intestinal research Vol.16 No.1
발행연도
2018.1
수록면
90 - 98 (9page)

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Background/Aims: To determine the prevalence of glycoprotein B (gB), glycoprotein N (gN), and glycoprotein H (gH) genotypes of human cytomegalovirus (HCMV) superimposed on ulcerative colitis (UC) patients in Japan. Methods: Four archivedstool samples and 7-archived extracted DNA from stool samples of 11 UC patients with positive multiplex polymerase chainreaction (PCR) results for HCMV were used UL55 gene encoding gB, UL73 gene encoding gN, and UL75 gene encoding gH were identified by PCR. Genotypes of gB and glycoprotein N were determined by sequencing. Results: Among 11 samples, 8samples were amplified through PCR. gB, gN, and gH genotypes were successfully detected in 3 of 8 (37.5%), 4 of 8 (50%), and8 of 8 (100%), respectively. The distribution of gB and gN genotypes analyzed through phylogenetic analysis were as follows:gB1 (2/3, 66.7%), gB3 (1/3, 33.3%), gN3a (2/4, 50%), and gN3b (2/4, 50%). Other gB genotypes (gB2 and gB4) and gN genotypes(gN1, gN2, and gN4) were not detected in this study. Out of successfully amplified 8 samples of gH genotype, gH1 and gH2 weredistributed in 12.5% and 75% samples, respectively. Only 1 sample revealed mixed infection of gH genotype. The distribution ofgH1 and gH2 differed significantly (1:6, P <0.05) in UC patients. The distribution of single gH genotype also revealed significantdifference in UC patients who were treated with immunosuppressive drug (P <0.05). Conclusions: In this study, gB1, gN3, andgH2 gene were determined as the most frequently observed genotypes in UC patients, which suggest that there might be an association between these genotypes of HCMV and UC.

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