Esculetin Inhibits the Survival of Human Prostate Cancer Cells by Inducing Apoptosis and Arresting the Cell Cycle

Kader Turkekul; R. Dilsu Colpan; Talha Baykul; Mehmet D. Ozdemir; Suat Erdogan

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자료유형: 학술저널

저자정보: Kader Turkekul (Trakya University) R. Dilsu Colpan (Trakya University) Talha Baykul (Trakya University) Mehmet D. Ozdemir (Trakya University) Suat Erdogan (Trakya University)

저널정보: 대한암예방학회 대한암예방학회지 대한암예방학회지 제23권 제1호

발행연도: 2018.1

수록면: 10 - 17 (8page)

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Background: Prostate cancer (PCa) is one of the most important causes of death in men and thus new therapeutic approaches are needed. In this study, antiproliferative and anti-migration properties of a coumarin derivative esculetin were evaluated. Methods: Human PCa cell lines PC3, DU145, and LNCaP were treated with various concentrations of esculetin for 24 to 72 hours, and cell viability was determined by the MTT test. Cell cycle and apoptosis were analyzed by using cell-based cytometer. Gene expression levels were assessed by reverse transcription and quantitative real-time PCR, cell migration was determined by the wound healing assay. The protein expression was measured by Western blotting. Results: Esculetin inhibited cell proliferation in a dose- and time-dependent manner. Cell migration was inhibited by esculetin treatment. Administration of esculetin significantly reduced the cells survival, induced apoptosis and caused the G1 phase cell cycle arrest shown by image-based cytometer. The induced expression of cytochrome c, p53, p21 and p27, and down-regulated CDK2 and CDK4 may be the underlying molecular mechanisms of esculetin effect. Esculetin suppressed phosphorylation of Akt and enhanced protein expression of tumor-suppressor phosphatase and tensin homologue. Conclusions: Our findings showed that the coumarin derivative esculetin could be used in the management of PCa. However, further in vivo research is needed. (J Cancer Prev 2018;23:10-17)

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참고문헌 신청

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