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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1,107 - 1,115 (9page)
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Zinc finger protein 703 (ZNF703) is a putative oncogene in patients with the luminal B molecular subtype of breast cancer. Although the exact function of ZNF703 protein remains largely unknown, its expression and regulation have been implicated in several physiological and pathological processes. In the current study, for the first time, we identified and characterized the human ZNF703 gene promoter region. As a means of characterizing the transcription elements required for expression of ZNF703 protein at different stages, we cloned the promoter region of ZNF703 then created chimeric reporter plasmids for use in luciferase assays. A progressive deletion analysis of the ZNF703 gene’s 5′ and 3′ -flanking regions revealed that the core promoter is located in a 256-bp region ranging from nt-539 to nt-283. Next, we examined the effects of sitespecific mutations and treatment with mithramycin A to identify the functional Sp1 binding site, which was found to be located in a 447 bp region that ranged from nt-509 to nt-76, displayed the characteristics of a CpG island, and overlapped with the promoter region. In conclusion, our data suggest that ZNF703 transcription is regulated by transcription factor Sp1. This finding should facilitate future studies of the mechanism which regulates expression of this important gene.
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참고문헌 (42)
참고문헌 신청
Bazarov AV / 2011 / Who is in the driver’s seat in 8p12amplifications? ZNF703 in luminal B breast tumo / Breast Cancer Res 13:308
Briggs MR / 1986 / Purification and biochemical characterization of the promoter- specific transcription factor, Sp1 / Science 234:47~52
Bucher P / 1990 / Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences / J Mol Biol 212:563~578
Castro-Rivera E / 2001 / Estrogen regulation of cyclin D1 gene expression in ZR-75 breast cancer cells involves multiple enhancer elements / J Biol Chem 276:30853~30861
Chin K / 2006 / Genomic and transcriptional aberrations linked to breast cancer pathophysiologies / Cancer Cell 10:529~541
Briggs MR / 1986 / Purification and biochemical characterization of the promoter- specific transcription factor, Sp1 / Science 234:47~52
Bucher P / 1990 / Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences / J Mol Biol 212:563~578
Castro-Rivera E / 2001 / Estrogen regulation of cyclin D1 gene expression in ZR-75 breast cancer cells involves multiple enhancer elements / J Biol Chem 276:30853~30861
Chin K / 2006 / Genomic and transcriptional aberrations linked to breast cancer pathophysiologies / Cancer Cell 10:529~541
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