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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 343 - 349 (7page)
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초록· 키워드
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Background Krüppel–like factor 10 (KLF10) belongs to the Sp1-like transcription factor family, which plays an important role in many directions, e.g., cell proliferation, apoptosis, and differentiation. Its 5′ upstream regions are conserved across mammalian species. However, the regulatory mechanism has not been elucidated yet. Objective Nonetheless the basal transcriptional regulation mechanisms of these regions are unknown. Here, we characterized it which is indispensable for the basal transcription of the Klf10 gene. Methods Seven deletions of 5′ upstream DNA fragments from the 10 kb mKlf10 genomic DNA were produced by PCR and cloned into the upstream of the luciferase (Luc) reporter gene in the pGL3 basic plasmid. Result The luciferase reporter assay showed that the DNA sequence at positions from −101 to +68 was required for a principle activity in the promoter of mKlf10 gene, in which transcriptional factor binding motifs, one JunB and two Sp1 sites, are included. Mutations at the sequence of JunB motif, but not at the two Sp1, abrogated the promoter activity completely, suggesting the indispensable role of JunB site for basal transcription of mKlf10 gene. Moreover, electrophoretic mobility and supershift assays (EMSA) uncovered that JunB protein bound to this region specifically. Conclusion Taken together, our study revealed that the JunB but not Sp1 at mKlf10 promoter functions as a positive basic factor for the transcriptional activity of the gene.
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참고문헌 (29)
참고문헌 신청
Azizkhan JC / 1993 / Transcription from TATA-less promoters : dihydrofolate reductase as a model / Crit Rev Eukaryot Gene Expr 3:229~254
Chang-Joon Bae / 2013 / Phthalic anhydride-induced skin inflammation is augmented in KLF10-deficient mice / Journal of Dermatological Science 71(3):221~224
Herdis Bender / 2004 / TIEG1 facilitates transforming growth factor-?-mediated apoptosis in the oligodendroglial cell line OLI-neu / Journal of Neuroscience Research 75(3):344~352
Sabine F. Bensamoun / 2006 / TGFβ inducible early gene-1 knockout mice display defects in bone strength and microarchitecture / Bone 39(6):1244~1251
James J. Bieker / 2001 / Krüppel-like Factors: Three Fingers in Many Pies / Journal of Biological Chemistry 276(37):34355~34358
Chang-Joon Bae / 2013 / Phthalic anhydride-induced skin inflammation is augmented in KLF10-deficient mice / Journal of Dermatological Science 71(3):221~224
Herdis Bender / 2004 / TIEG1 facilitates transforming growth factor-?-mediated apoptosis in the oligodendroglial cell line OLI-neu / Journal of Neuroscience Research 75(3):344~352
Sabine F. Bensamoun / 2006 / TGFβ inducible early gene-1 knockout mice display defects in bone strength and microarchitecture / Bone 39(6):1244~1251
James J. Bieker / 2001 / Krüppel-like Factors: Three Fingers in Many Pies / Journal of Biological Chemistry 276(37):34355~34358
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