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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 39 - 50 (12page)
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BACKGROUND: Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits. METHODS: The tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxylin–eosin staining, Masson trichrome staining, Safranin O staining, DAPI staining, scanning electronic microscopy (SEM), and biocompatibility tests were used to evaluate the treatment. The bioengineered trachea and control trachea were then implanted into allogeneic rabbits for 30 days. The structural and functional analyses were performed after transplantation. RESULTS: The biomechanical tests demonstrated that the biomechanical properties of the decellularized tracheas decreased and that genipin improved them (p\0.05). The histological staining results revealed that most of the mucosal epithelial cells were removed and that the decellularized trachea had lower immunogenicity than the control group. The analysis of SEM revealed that the decellularized trachea retained the micro- and ultra-structural architectures of the trachea and that the matrices cross-linked with genipin were denser. The biocompatibility evaluation and in vivo implantation experiments showed that the decellularized trachea treated with the DEM had better biocompatibility than that treated with the TPM and that immunogenicity in the cross-linked tissues was lower than that in the uncross-linked tissues (p\0.05). CONCLUSIONS: Compared with the trachea treated with the TPM, the rabbit trachea processed by the DEM had better biocompatibility and lower immunogenicity, and its structural and mechanical characteristics were effectively improved after the genipin treatment, which is suitable for engineering replacement tracheal tissue.
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참고문헌 (39)
참고문헌 신청
Sukumaran R / 2014 / Extramedullary plasmacytoma of the trachea / Head Neck Pathol 8:220~224
Kojima K / 2014 / Tissue engineering in the trachea / Anat Rec(Hoboken) 297:44~50
Luo X / 2013 / Long-term functional reconstruction of segmental tracheal defect by pedicled tissue-engineered trachea in rabbits / Biomaterials 34:3336~3344
Elliott MJ / 2012 / Stem-cell-based, tissue engineered tracheal replacement in a child : a 2-year follow-up study / Lancet 380:994~1000
Ott LM / 2011 / Overview of tracheal tissue engineering : clinical need drives the laboratory approach / Ann Biomed Eng 39:2091~2113
Kojima K / 2014 / Tissue engineering in the trachea / Anat Rec(Hoboken) 297:44~50
Luo X / 2013 / Long-term functional reconstruction of segmental tracheal defect by pedicled tissue-engineered trachea in rabbits / Biomaterials 34:3336~3344
Elliott MJ / 2012 / Stem-cell-based, tissue engineered tracheal replacement in a child : a 2-year follow-up study / Lancet 380:994~1000
Ott LM / 2011 / Overview of tracheal tissue engineering : clinical need drives the laboratory approach / Ann Biomed Eng 39:2091~2113
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