지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
1-Deoxynojirimycin (DNJ), an alpha-glucosidase inhibitor, has been used to prevent or treat type 2 diabetes. Low amounts of DNJ are found in mulberry leaf; however, a methodology is necessary to enhance the DNJ content of mulberry leaf extract (MLE) since increasing the amounts of DNJ is required for the prevention and treatment of type 2 diabetes. In this study, the DNJ content of MLE was increased through the optimization of the conditions for MLE fermentation by Lactobacillus plantarum SG-053, using response surface methodology (RSM). By single factor testing, the optimal conditions were identified as an inoculum concentration of 1%(v/v), an MLE concentration of 3°Bx, and no agitation. Based on a Plackett-Burman design, the following factors were considered to majorly affect the DNJ content in the MLE fermentation product: the initial pH, fermentation temperature, and fermentation time. By response surface methodology, the optimal conditions for MLE fermentation was found to have an initial pH of 5.5, a fermentation temperature of 31.9oC, and a fermentation time of 34 h. Under these optimal fermentation conditions, the DNJ content in MLE increased 3.59 times, or from 23.85 to 85.54 μg/mL.
목차
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참고문헌 (23)
참고문헌 신청
Choi J / 2018 / Alpha-glucosidase inhibitory and antioxidant activities of lactic acid bacteria isolated from Korean traditional fermented foods / Proceedings of 2018 KoSFoST International Symposium and Annual Meeting :585
Fang M, Huang AQ, Zhang YQ / 2012 / The correlation between 1-deoxynojirimycin content and α-glucosidase inhibitory activity in the bark ethanol extract from Ramulus mori / iCBEB2012. IEEE Xplore :1795~1798
정종훈 / 2014 / Enhancement of 1-Deoxynojirimycin Content and α-Glucosidase Inhibitory Activity in Mulberry Leaf Using Various Fermenting Microorganisms Isolated from Korean Traditional Fermented Food / Biotechnology and Bioprocess Engineering 19(6):1114~1118
Ji T / 2016 / Identification and determination of the polyhydroxylated alkaloids compounds with α-glucosidase inhibitor activity in mulberry leaves of different origins / Molecules 21:206
Jiang YG / 2014 / Improved 1-Deoxynojirimycin(DNJ)production in mulberry leaves fermented by microorganism / Braz. J. Microbiol 45:721~729
Fang M, Huang AQ, Zhang YQ / 2012 / The correlation between 1-deoxynojirimycin content and α-glucosidase inhibitory activity in the bark ethanol extract from Ramulus mori / iCBEB2012. IEEE Xplore :1795~1798
정종훈 / 2014 / Enhancement of 1-Deoxynojirimycin Content and α-Glucosidase Inhibitory Activity in Mulberry Leaf Using Various Fermenting Microorganisms Isolated from Korean Traditional Fermented Food / Biotechnology and Bioprocess Engineering 19(6):1114~1118
Ji T / 2016 / Identification and determination of the polyhydroxylated alkaloids compounds with α-glucosidase inhibitor activity in mulberry leaves of different origins / Molecules 21:206
Jiang YG / 2014 / Improved 1-Deoxynojirimycin(DNJ)production in mulberry leaves fermented by microorganism / Braz. J. Microbiol 45:721~729
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