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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 123 - 131 (9page)
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Background The 16q23.1 tumor suppressor gene (TSG) of ADAMTS18 has been identified to be aberrant methylated in clear cell renal cell carcinoma (ccRCC), and there still exists an unclear situation between its methylation and the progression of ccRCC. Objective To analyze the biological function and mechanism of ADAMTS18 gene in the tumorigenesis and progression of ccRCC. Methods We examined ADAMTS18 gene methylation using methylation- specific polymerase chain reaction (MSP) in 92 ccRCC primary tumors from September 2017 to May 2018. Using reverse transcriptase PCR (RT-PCR) and immunohistochemical (IHC) assay, the relative expression level of ADAMTS18 was measured in the representative tumor samples with their adjacent normal tissues. Meanwhile, colony formation, cell viability, wound healing, transwell chamber, flow cytometry, and PI staining were performed to confirm the tumor-suppressive function and mechanism of ADAMTS18 gene. Results Aberrant methylation was further detected in 47 of the 92 (51.1%) primary tumors and in 8 of the 92 (8.7%) adjacent normal tissues (p < 0.05). Due to the phenomenon of aberrant methylation, ectopic low-level expression of ADAMTS18 gene could result in the promotion of tumorigenesis and progression in ccRCC. Conclusion The aberrantly methylated ADAMTS18 gene may be involved in the tumorigenesis and progression of ccRCC.
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참고문헌 (29)
참고문헌 신청
Ben X / 2015 / Hypermethylation of the 16q23. 1 tumor suppressor gene ADAMTS18 in clear cell renal cell carcinoma / Int J Mol Sci 16:1051~1065
Brittany NL / 2018 / The role of DNA methylation in renal cell carcinoma / Mol Diagn Ther 22:431~442
Chunhong L / 2015 / OPCML is frequently methylated in human colorectal cancer and its restored expression reverses EMT via downregulation of smad signaling / Am J Cancer Res 5:1635~1648
Dalya A / 2020 / The secreted protease Adamts18 links hormone action to activation of the mammary stem cell niche / Nat Commun 11:1571
Hongchuan J / 2007 / Epigenetic identifcation of ADAMTS18 as a novel 16q23. 1 tumor suppressor frequently silenced in esophageal, nasopharyngeal and multiple other carcinomas / Oncogene 26:7490~7498
Brittany NL / 2018 / The role of DNA methylation in renal cell carcinoma / Mol Diagn Ther 22:431~442
Chunhong L / 2015 / OPCML is frequently methylated in human colorectal cancer and its restored expression reverses EMT via downregulation of smad signaling / Am J Cancer Res 5:1635~1648
Dalya A / 2020 / The secreted protease Adamts18 links hormone action to activation of the mammary stem cell niche / Nat Commun 11:1571
Hongchuan J / 2007 / Epigenetic identifcation of ADAMTS18 as a novel 16q23. 1 tumor suppressor frequently silenced in esophageal, nasopharyngeal and multiple other carcinomas / Oncogene 26:7490~7498
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