지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 361 - 369 (9page)
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초록· 키워드
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Background Recurrent pregnancy loss (RPL) refers to two or more consecutive spontaneous abortion before 24 weeks of gestation, representing 1% of couples of childbearing age. Epigenetic factors including dysregulation of DNA methylation of some genes may play a role in RPL. Objective To identify RPL related genes modulated by DNA methylation expressed in decidua and blood. Methods Three decidua samples each from RPL patients and normal controls were recruited to perform genome-wide bisulfite sequencing (GWBS) and transcriptome sequencing. Based on the above results, 22.52 kb of differential methylation regions (DMRs) from 17 genes were verified by bisulfite sequencing PCR at specific region (Hi-MethylSeq) in another 15 decidua (7RPL vs. 8 Controls) and 13 blood (5RPL vs. 8 Controls) samples. Results 23 genes showed significantly differential cytosine methylation status and distinct expression level between PRL patients and healthy controls synergistically. Three signaling pathways were found to be shared between genes with both hypomethylated differential methylation regions (DMR) and upregulated differential gene expression (DGE). The results from Hi-MethylSeq showed that the hypermethylation of SGK1 in both blood and decidua samples in RPL patients, which was consistent to its lower expression in endometrium reported earlier. SGK3 and CREB5 also showed modulated methylation level in RPL decidua. Conclusion Our finding supported that aberrant methylation of SGK1 and CREB5 could be a cause of the dysregulation of these gens in the endometrium, which is one of cause of reproductive failure. The function of SGK3 in reproduction system deserves further investigation.
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참고문헌 (20)
참고문헌 신청
Akalin AKM / 2012 / A comprehensive R package for the analysis of genome-wide DNA methylation profles / Genome Biol 13:R87
Andrews FKSR / 2011 / Bismark : a fexible aligner and methylation caller for Bisulfte-Seq applications / Bioinformatics 27:2
Arias-Sosa LA / 2018 / Genetic and epigenetic variations associated with idiopathic recurrent pregnancy loss / J Assist Reprod Genet 35:355~366
Ashburner M / 2000 / Gene ontology : tool for the unifcation of biology. The Gene Ontology Consortium / Nat Genet 25:25~29
Brunet A / 2001 / Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1(FOXO3a) / Mol Cell Biol 21:952~965
Andrews FKSR / 2011 / Bismark : a fexible aligner and methylation caller for Bisulfte-Seq applications / Bioinformatics 27:2
Arias-Sosa LA / 2018 / Genetic and epigenetic variations associated with idiopathic recurrent pregnancy loss / J Assist Reprod Genet 35:355~366
Ashburner M / 2000 / Gene ontology : tool for the unifcation of biology. The Gene Ontology Consortium / Nat Genet 25:25~29
Brunet A / 2001 / Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1(FOXO3a) / Mol Cell Biol 21:952~965
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