지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Methylmalonyl-CoA epimerase (MMCE) is an enzyme involved in the carbon capturing 3-HP/4-HB cycle, by catalyzing the reaction of converting (S)-methylmalonyl-CoA to (R)-methylmalonyl-CoA. In this study, we overexpressed MMCE from Metallosphaera sedula (MsMMCE) and purified the protein to homogeneity by Ni-NTA affinity and size-exclusion chromatography. The MsMMCE protein was crystallized using the hanging-drop vapor-diffusion method in the presence of 20% (w/v) polyethylene glycol 3350, and 0.2 M ammonium nitrate at 293 K. X-ray diffraction data were collected to a maximum resolution of 2.1 A. The MsMMCE crystals belong to the space group P21 with unit cell parameters a = 51.87 A, b = 79.00 A, c = 106.09, α = γ = 90.0°, β = 95.80°. With six molecules of MsMMCE per an asymmetric unit, the crystal volume per unit of protein mass is 2.33 A3 Da?1, which corresponds to a solvent content of approximately 47.13%.
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참고문헌 (23)
참고문헌 신청
Antranikian, G. / 1982 / Characterization of ATP citrate lyase from Chlorobium limicola / J Bacteriol 152:1284~1287
Arctic Climate Impact Assessment / 2004 / Impacts of a warming Artic / Cambridge University Press
Berg, I. A. / 2007 / A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea / Science 318:1782~1786
Berg, I. A. / 2010 / Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota / Microbiology(Reading) 156:256~269
Chalmers, R.A. / 1982 / Organic acids in man Ch.11 / Springer
Arctic Climate Impact Assessment / 2004 / Impacts of a warming Artic / Cambridge University Press
Berg, I. A. / 2007 / A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea / Science 318:1782~1786
Berg, I. A. / 2010 / Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota / Microbiology(Reading) 156:256~269
Chalmers, R.A. / 1982 / Organic acids in man Ch.11 / Springer
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