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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Homoserine O-succinyltransferase from Escherichia coli (EcHST) is the first enzyme in methionine biosynthesis and it catalyzed the transfer of succinyl-group from succinyl-CoA to L-homoserine. EcHST has reported to be regulated by feedback inhibition and it controls the bacterial growth due to its instability. To improve the EcHST for industrial application, the protein was overexpressed and purified to homogeneity by Ni-NTA affinity and size-exclusion chromatography. The EcHST protein was crystallized using hanging-drop vapor-diffusion method in the presence of 25% polyethylene glycol 3350, 0.1 M HEPES, pH 7.5, and 0.1 M magnesium chloride at 295 K. X-ray diffraction data were collected to a maximum resolution of 1.7 Å. The EcHST crystals belonged to the space group P212121 with unit cell parameters a = 76.35 Å, b = 81.03 Å, c = 98.09, α = β = γ = 90.0°. With two molecules of EcHST per asymmetric unit, the crystal volume per unit of protein mass was 2.12 Å3 Da-1, which correspond to a solvent content was approximately 42.15%.
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참고문헌 (20)
참고문헌 신청
Bestel-Corre, G / 2014 / Recombinant enzyme with altered feedback sensitivity (Google Patents)
Born, T. L. / 1999 / Enzyme-catalyzed acylation of homoserine : mechanistic characterization of the Escherichia coli metAencoded homoserine transsuccinylase / Biochemistry 38:14416~14423
Born, T. L. / 2000 / Enzyme-catalyzed acylation of homoserine : mechanistic characterization of the Haemophilus influenzae met2-encoded homoserine transacetylase / Biochemistry 39:8556~8564
Duclos, B. / 1989 / Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli / Nucleic Acids Res 17:2856
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Born, T. L. / 1999 / Enzyme-catalyzed acylation of homoserine : mechanistic characterization of the Escherichia coli metAencoded homoserine transsuccinylase / Biochemistry 38:14416~14423
Born, T. L. / 2000 / Enzyme-catalyzed acylation of homoserine : mechanistic characterization of the Haemophilus influenzae met2-encoded homoserine transacetylase / Biochemistry 39:8556~8564
Duclos, B. / 1989 / Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli / Nucleic Acids Res 17:2856
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
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