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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 63 - 66 (4page)
- DOI
- 10.34184/kssb.2021.9.4.63
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초록· 키워드
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A tumor suppressor protein PTPN14 interacts with the oncoprotein E7 of human papillomaviruses, leading to its proteasomal degradation. Introduction of F1044S, G1055Q, and E1095A mutations into its phosphatase domain restored the antitumor activity by impairing the interaction with E7, showing the therapeutic potential of this mutant form against human papillomavirus-involved cancers. In this study, the phosphatase domain of PTPN14 containing the three mutations was produced in an Escherichia coli expression system, purified using Ni-NTA affinity and size exclusion chromatographies, and then crystallized. X-ray diffraction data with a maximum resolution of 1.50 A were successfully collected, and a preliminary diffraction analysis was conducted. Our crystals belonged to the P21 space group with unit cell parameters of a = 43.9 A, b = 74.3 A, c = 47.8 A, and β = 102.0°. The asymmetric unit contains one protein molecule with a 45% solvent content and a 2.2 A3/Da Matthews coefficient.
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참고문헌 (16)
참고문헌 신청
Bray, F. / 2018 / Global cancer statistics 2018 : GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries / CA Cancer J Clin 68:394~424
Huang, J. M. / 2013 / YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14 / Oncogene 32:2220~2229
Lee, H. / 2015 / Mining the function of protein tyrosine phosphatases in health and disease / Semin Cell Dev Biol 37:66~72
Hye Seon Lee / 2021 / Molecular Analysis of the Interaction between Human PTPN21 and the Oncoprotein E7 from Human Papillomavirus Genotype 18 / Molecules and Cells 44(1):26~37
Mello, S. S. / 2017 / A p53 super-tumor suppressor reveals a tumor suppressive p53-Ptpn14-Yap axis in pancreatic cancer / Cancer Cell 32:460.e466~473.e466
Huang, J. M. / 2013 / YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14 / Oncogene 32:2220~2229
Lee, H. / 2015 / Mining the function of protein tyrosine phosphatases in health and disease / Semin Cell Dev Biol 37:66~72
Hye Seon Lee / 2021 / Molecular Analysis of the Interaction between Human PTPN21 and the Oncoprotein E7 from Human Papillomavirus Genotype 18 / Molecules and Cells 44(1):26~37
Mello, S. S. / 2017 / A p53 super-tumor suppressor reveals a tumor suppressive p53-Ptpn14-Yap axis in pancreatic cancer / Cancer Cell 32:460.e466~473.e466
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