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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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초록· 키워드
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Spermidine synthase (SpdS) is an aminopropyltransferase that transfers an aminopropyl moiety from decarboxylated S-adenosylmethionine to a variety of polyamines. SpdS enzymes convert putrescine to spermidine and its byproduct methylthioadenosine. In this study, we have overexpressed an N-terminal His6-tagged SpdS from the fungal species Kluyveromyces lactis; it was overexpressed, purified and crystallized to obtain X-ray diffraction data at a high resolution of 1.9 A. The K. lactis SpdS crystal belongs to the space group P212121 with the following unit cell parameters: a = 65.252 A, b = 98.180 A, c = 102.134 A, and α = β = γ = 90°. There are two molecules in the asymmetric unit.
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참고문헌 (17)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Dufe, V. T. / 2005 / Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase / FEBS Lett 579:6037~6043
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Fontecave, M. / 2004 / S-adenosylmethionine : nothing goes to waste / Trends Biochem Sci 29:243~249
Guédez, G. / 2019 / Crystal structure of dimeric Synechococcus spermidine synthase with bound polyamine substrate and product / Biochem J 476:1009~1020
Dufe, V. T. / 2005 / Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase / FEBS Lett 579:6037~6043
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Fontecave, M. / 2004 / S-adenosylmethionine : nothing goes to waste / Trends Biochem Sci 29:243~249
Guédez, G. / 2019 / Crystal structure of dimeric Synechococcus spermidine synthase with bound polyamine substrate and product / Biochem J 476:1009~1020
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