Effects of Amniotic Membrane Extract on the Hyperplastic Response of the Middle Ear Mucosa in a Bacterially-Induced Otitis Media Rat Model: A Preliminary Study

박주현; 김희복; 고서현; 김보해; 임윤성; 박석원; 송재준; 조창건

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자료유형: 학술저널

저자정보: 박주현 (동국대학교) 김희복 (동국대학교) 고서현 (동국대학교 의과학연구소) 김보해 (동국대학교) 임윤성 (동국대학교) 박석원 (동국대학교) 송재준 (고려대학교) 조창건 (동국대학교)

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제13권 제4호

발행연도: 2020.1

수록면: 381 - 388 (8page)

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Objectives. Human amniotic membrane extract (AME) is known to contain numerous bioactive factors and anti-inflammatory substances. However, the anti-inflammatory effects of AME on the middle ear (ME) mucosa are unclear. This study assessed the effects of AME on the growth of the ME mucosa in response to bacterially-induced otitis media (OM). Methods. OM was induced by inoculating nontypeable Haemophilus influenzae (NTHi) into the ME cavity of rats. ME mucosal explants were cultured in AME concentrations of 0, 5, 10, or 50 μg/mL. The area of explant outgrowth was measured in culture and analyzed at 1, 3, 5, and 7 days after explantation. The expression of Ki-67, mucin 5AC (MUC5AC), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in the explants was also evaluated using quantitative polymerase chain reaction (PCR) and immunocytochemistry (ICC). Results. The NTHi-induced ME mucosa growth increased gradually over the 7-day culture period in all explants at different AME concentrations. There was a trend for mucosal growth inhibition at higher concentrations of AME, although the growth was not significantly different among the groups until day 5. The ME mucosal explants treated with the 50 μg/mL concentration of AME showed significantly suppressed growth on postexplantation day 7 compared with other explants on the same day. PCR and ICC staining revealed that the expression of Ki-67, MUC5AC, TNF-α, and IL-10 further decreased in the explants with higher concentrations of AME than in those with lower concentrations of AME. Conclusion. Our results showed that higher concentrations of AME reduced the mucosal proliferative response in bacterial OM in rats. These findings provide evidence that AME has an influence on the inflammatory and proliferative responses to NTHi infection in ME mucosa.

#Otitis Media #Amniotic Membrane #Haemophilus Influenzae #Rat

참고문헌 (8)

참고문헌 신청

Coticchia JM / 2013 / New paradigms in the pathogenesis of otitis media in children / Front Pediatr 1:52

Rovers MM / 2004 / Otitis media / Lancet 363(9407):465~473

Infante-Rivard C / 1993 / Otitis media in children : frequency, risk factors, and research avenues / Epidemiol Rev 15(2):444~465

Klein JO / 2000 / The burden of otitis media / Vaccine 19(Suppl 1):S2~S8

Al Khabori M / 2004 / The prevalence and causes of hearing impairment in Oman : a community-based cross-sectional study / Int J Audiol 43(8):486~492

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